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以甘蔗栽培品种ROC22和含有野生斑茅蔗血缘的BC2-32为材料,通过乙炔还原法评价了甘蔗不同组织的固氮能力。结果显示,在分蘖期,ROC22根系的固氮酶活性较高,可能是联合固氮的主要器官;在伸长期,利用以蔗糖为碳源的LGI、SNX和SH培养基培养,甘蔗叶片的固氮酶活性最高;以苹果酸为碳源的JNFb和Mal处理,积累一定糖分的+4和+5茎的固氮酶活性较高,暗示了叶片主要定殖以蔗糖为碳源的内生固氮菌,是伸长期固氮效率最高的部位,其次为蔗茎。ROC22根系在SH培养条件下的固氮酶活性比BC2-32高25倍,说明ROC22根系中含有某些优势内生固氮菌;而两种甘蔗不同叶和蔗茎的固氮酶活性在伸长期表现较为相似的趋势,说明ROC22和BC2-32中定殖的主要是以蔗糖为碳源,较为保守且专性较强的内生固氮菌。
The sugarcane Cultivars ROC22 and the BC2-32 containing the blood of wild broomcorn were used as materials to evaluate the nitrogen fixation ability of different tissues of sugarcane by acetylene reduction method. The results showed that at the tillering stage, the root system of ROC22 had higher nitrogenase activity and might be the main organ for the combination of nitrogen fixation. During the elongation stage, the nitrogenase activity of sugarcane leaves was cultured in LGI, SNX and SH medium with sucrose as carbon source The highest nitrogenase activity of +4 and +5 stems, which accumulated some sugar, implied that endophytic diazotrophs with sucrose as carbon source were mainly colonized by leaves of maize, Long-term nitrogen fixation efficiency of the site, followed by cane stems. ROC22 root activity in SH culture medium nitrogenase activity than BC2-32 25 times higher, indicating that ROC22 root system contains some of the advantages of endophytic diazotrophs; and two kinds of sugar cane leaf and cane nitrogenase activity in the elongation phase of the more A similar trend indicates that the colonized ROC22 and BC2-32 are mainly endophytic azotobacter with sucrose as carbon source, more conservative and more obligate.