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目的:观察并比较不同提取工艺、不同实验条件银翘散主要药物体外直接抑制流感病毒作用,进行银翘散体外抑病毒方法学研究。方法:采用血凝试验对银翘散主要药物提取物不同溶剂、不同时间点进行体外抗甲型流感病毒的效价测定。结果:银翘散主要药物提取物各方法提取均可直接抑制甲型流感病毒FM1株的活性,作用时间从1h可持续到24h,其中银翘散30%醇提物抑制流感病毒增殖的作用最强;各组药物在最大不凝血浓度均能抑制病毒的增殖。结论:银翘散提取物可有效抑制甲型流感病毒。
OBJECTIVE: To observe and compare different extraction processes. The main drugs of Yinqiao Powder directly inhibit the effect of influenza virus in vitro and in vitro. Methods: Hemagglutination test was used to determine the titer of anti-influenza A virus in vitro in different solvents and at different time points. Results: The extract of Yinqiao Powder’s main drug extracts could directly inhibit the activity of FM1 strain of influenza A virus, the duration of action was from 1h to 24h, and the effect of 30% ethanol extract of Yinqiaoqiao on the inhibition of influenza virus proliferation Strong; each group of drugs in the maximum non-coagulation concentration can inhibit the proliferation of the virus. Conclusion: Yinqiao powder extract can effectively inhibit influenza A virus.