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初步探讨了利用高盐抑制受精卵第2极体(PB2)的释放的方法诱导太平洋牡蛎(Crassostrea gigas)三倍体。水温25℃条件下,分别进行不同高盐处理(盐度梯度为40、45、50、55、60、65、70、75、80)、不同处理时机(受精卵出现第一个PB1,30%和50%PB1,出现第一个PB2,50%PB2)和不同持续处理时间(10~25min)的实验,通过胚胎孵化率、三倍体诱导率及综合评价指数的分析表明,高盐诱导太平洋牡蛎三倍体的最适方案为:当50%受精卵出第一极体时,以盐度为65的高盐海水处理受精卵20min,三倍体诱导率最高达65.53%。
Preliminary studies on the use of high salt to inhibit the release of the second polar body (PB2) of fertilized eggs induced Pacific triploid oyster (Crassostrea gigas). Under the conditions of water temperature 25 ℃, different high salt treatments (salinity gradient 40, 45, 50, 55, 60, 65, 70, 75 and 80) And 50% PB1, the first PB2, 50% PB2 appeared) and different duration of treatment (10 ~ 25min). The analysis of embryo hatching rate, triploid induction rate and comprehensive evaluation index showed that high salt induced the Pacific Ocean The optimal plan of oyster triploid is: when 50% of fertilized eggs are in the first polar body, the fertilized eggs are treated with high salinity seawater with a salinity of 65 for 20 minutes, the highest diploid induction rate is 65.53%.