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以马铃薯品种费乌瑞它微型薯为外植体,采用农杆菌介导法将BADH耐盐基因导入马铃薯中,优化了影响遗传转化的因素,包括激素浓度、脱菌抗生素浓度、共培养时间、筛选剂浓度等。共获得30株PPT抗性植株,PCR检测10株为阳性植株,Southern检测4株为阳性植株。生理检测表明植株在0.7%NaCl浓度下耐盐性相对较高。证明外源基因已经整合到植物的基因组上。
The potato variety Fawaii miniature potato was used as explant, and BADH salt-tolerant gene was introduced into potato by Agrobacterium-mediated method, and the factors affecting genetic transformation were optimized, including hormone concentration, concentration of antibiotic antibiotic, co-culture time, Screening agent concentration and so on. A total of 30 PPT resistant plants were obtained, 10 were positive by PCR and 4 by Southern. Physiological tests showed that the salt tolerance of plants was relatively high at 0.7% NaCl concentration. Proved that the foreign gene has been integrated into the plant’s genome.