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应用Dig标记的cDNA探针对5l例膀胱移行细胞癌石蜡切片进行C－myc、Ha－ras癌基因mRNA的原位杂交检测。结果显示，C－mycmRNA阳性率为62．75％（32／51），Ha－ras为70．6％（36／51）。两者的阳性率和阳性强度随肿瘤恶性程度的升高而增加。在临床分期较高的肿瘤中两者的阳性率亦明显增高。上述结果揭示，C－myc、Ha－ras癌基因在人膀胱移行细胞癌中的表达增强与膀胱癌的恶性程度及其肿瘤生物学行为有关，两者是判定膀胱癌恶性程度及预后有价值的指标，对制定合理的治疗方案可提供有意义的帮助。
Dig-labeled cDNA probes were used to detect the C-myc and Ha-ras oncogene mRNA in 5l bladder transitional cell carcinoma paraffin sections. The results showed that the positive rate of C-mycmRNA was 62.75% (32/51) and Ha-ras was 70.6% (36/51). Both the positive rate and positive intensity increased with the malignant degree of the tumor. The positive rate of the two in the higher clinical stage tumors was also significantly higher. The above results revealed that the enhanced expression of C-myc and Ha-ras oncogenes in human bladder transitional cell carcinoma is related to the malignant degree of bladder cancer and the biological behavior of the tumor, both of which are valuable in judging the malignancy and prognosis of the bladder cancer Indicators provide meaningful assistance in developing a rational treatment plan.