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目的将体外培养的人脐带间充质干细胞(hUCMSCs)与血管内皮生长因子(VEGF)联合植入脑缺血(MCAO)模型大鼠脑内,观察缺血区血管新生和神经功能缺损修复情况。方法选择75只SD大鼠分为MCAO组、hUCMSCs组、VEGF组和hUCMSCs+VEGF组,观察在缺血前、缺血后7d、14d、28d4个时间点改良神经功能评分(mNSS)变化及7d、14d、28d3个时间点免疫组织化学法检测脑缺血区域微血管密度(MVD)。结果在缺血后7d、14d、28d3个时间点mNSS评分:shamhUCMSCsorVEGF>hUCMSCs+VEGF(P<0.05),hUCMSCs组、VEGF组、hUCMSCs+VEGF组的新生血管密度均高于MCAO组(P<0.05),其中hUCMSCs+VEGF组微血管密度最高(P<0.01)。结论 hUCMSCs联合VEGF移植入MCAO模型大鼠脑内,可促进其缺血区血管新生和神经功能的恢复。
OBJECTIVE: To investigate the effects of hUCMSCs and vascular endothelial growth factor (VEGF) combined with hUCMSCs implanted in brain of cerebral ischemia rats (MCAO) on the repair of ischemic neovascularization and neurological deficits. Methods Seventy-five Sprague Dawley rats were divided into MCAO, hUCMSCs, VEGF and hUCMSCs + VEGF groups. The changes of mNSS score at 7d, 14d and 28d after ischemia and 7d , 14d, 28d three time points immunohistochemical detection of cerebral ischemia regional microvessel density (MVD). Results The neovascularization density of hUCMSCs group, VEGF group and hUCMSCs + VEGF group were higher than those of MCAO group at the time points of 7d, 14d and 28d (P <0.05) (P <0.05). The microvessel density of hUCMSCs + VEGF group was the highest (P <0.01). Conclusion The transplantation of hUCMSCs and VEGF into the brain of MCAO rats can promote the recovery of angiogenesis and neurological function in the ischemic area.