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背景:学习记忆是一个十分复杂的生物现象,参与学习记忆的物质以及与学习记忆有关的脑区虽然研究较多,但是,其机制仍没有完全阐明。目的:探讨不同学习记忆能力大鼠的不同脑区抗氧化酶活性的差异,以揭示学习记忆能力与特定脑区抗氧化酶活性的关系。设计:以实验动物为对象,完全随机设计实验。单位:济宁医学院生物化学教研室。材料:实验于2001-03/2004-01在济宁医学院生化教研室及苏州医学院完成。wistar大鼠40只,15月龄,雄性,体质量580～650g。干预:学习记忆能力的检测在MG-2型三等分辐射式迷宫中进行。大鼠受电击后直接逃至安全区为正确反应,连续10次有9次正确即达到了学会的标准,观察指标有达标所需反应次数及正确反应率。达标所需反应次数低于(或等于)40次者为学习能力好。否则为学习能力差。24h后重复检验,观察其记忆保持情况。连续3次均能正确反应者为记忆能力好,否则为记忆能力差。学习能力、记忆能力均好组(10只);学习能力、记忆能力均差组(12只)。其余被淘汰掉。主要观察指标:两组大鼠大脑皮质、小脑、纹状体、海马、下丘脑5个脑区超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性。结果:学习记忆能力均差的大鼠与学习记忆能力均好的大鼠比较:皮质、海马、纹状体超氧化物歧化酶活性显著降低(t=
Background: Learning and memory is a very complex biological phenomenon. Although there are many studies on the substances involved in learning and memory and the brain regions related to learning and memory, the mechanism is still not completely elucidated. OBJECTIVE: To investigate the differences of antioxidant enzyme activities in different brain regions of different learning and memory abilities in rats to reveal the relationship between learning and memory abilities and antioxidant enzyme activities in specific brain regions. Design: Experimental animals as the object, a completely random design experiment. Unit: Department of Biochemistry, Jining Medical College. MATERIALS: Experiments were performed at Department of Biochemistry, Jining Medical College and Suzhou Medical College from March 2001 to January 2004. Wistar rats 40, 15 months old, male, body weight 580 ~ 650g. Interventions: The learning and memory abilities were tested in the MG-2 trisectional radiation maze. Rats directly fled to the safe area by the electric shock for the correct response, 10 times in a row 9 times correctly to achieve the standard of learning to observe the indicators have the required number of reactions required and the correct response rate. The number of reactions required to achieve compliance is less than (or equal to) 40 times for learning ability. Otherwise poor learning ability. 24h after repeated testing to observe the memory retention. For 3 times in a row can be the correct response to good memory, or poor memory. Learning ability, memory ability are good group (10); learning ability, poor memory ability group (12). The rest is eliminated. MAIN OUTCOME MEASURES: The activities of superoxide dismutase, catalase and glutathione peroxidase in the cerebral cortex, cerebellum, striatum, hippocampus and hypothalamus in two groups were compared. RESULTS: Compared with rats with good learning and memory ability, the rats with poor learning and memory ability had significantly lower activities of cortical, hippocampal and striatal SOD (t =