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目的了解深圳市宝安区病毒性腹泻的感染情况。方法采用实时荧光定量–聚合酶链反应对2011年5月—2012年4月宝安区403例腹泻患者的粪便标本进行诺如病毒和轮状病毒的核酸检测。结果腹泻患者的病毒阳性率为37.22%,其中诺如病毒和轮状病毒的阳性率分别为20.35%和18.86%。诺如病毒和轮状病毒全年各月均有检出,诺如病毒在6—10月检出较多,轮状病毒秋冬季为发病高峰。儿童的病毒阳性率和轮状病毒检出率显著高于成人。结论诺如病毒和轮状病毒是辖区内病毒性腹泻的主要病原,应加强全部人群的病毒性腹泻监测工作。
Objective To understand the infection of viral diarrhea in Bao’an District of Shenzhen City. Methods Real-time fluorescence quantitative polymerase chain reaction (PCR) was used to detect the DNA of norovirus and rotavirus in 403 stool samples from diarrhea patients in Bao’an District from May 2011 to April 2012. Results The positive rate of virus in patients with diarrhea was 37.22%. The positive rates of norovirus and rotavirus were 20.35% and 18.86% respectively. Norovirus and rotavirus were detected throughout the year, Norovirus detected more from June to October, the rotavirus peak in autumn and winter. Children’s positive rate of virus and rotavirus were significantly higher than adults. Conclusions Norovirus and rotavirus are the main pathogens of viral diarrhea in their area, and the monitoring of viral diarrhea should be strengthened in all the population.