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目的:2012年6月,位于锦州市某地两名村民宰杀牛后,疑似感染炭疽,采集样本对其做分离培养、鉴定炭疽芽孢杆菌。方法:采集样本进行分离培养炭疽芽孢杆菌,通过镜检、噬菌体裂解试验、串珠试验等方法及荧光PCR法、普通PCR法鉴定。结果:3份患者手分泌物样本在荧光PCR法检测中均为阳性,在普通PCR法采用三对引物PagA、Cap及rpoB检测在923 bp,1242 bp,618 bp处出现特异性目的条带。结论:本次检测在短时间内顺利完成,为疫情防控工作提供有力的实验室支持;同时为及时治疗患者疾病争取了宝贵时间。
Objective: In June 2012, two villagers in Jinzhou City, after slaughtering cattle, suspected of being infected with anthrax. Samples were collected for isolation and culture, and B. anthracis was identified. Methods: Bacillus anthracis was isolated and cultured by microscopic examination, bacteriophage lysis test, beaded test and fluorescence PCR method, and identified by ordinary PCR. Results: The secretions from 3 patients were all positive by fluorescent PCR. Three pairs of primers PagA, Cap and rpoB were used to detect specific bands in 923 bp, 1242 bp and 618 bp. Conclusion: The test was successfully completed in a short period of time, providing strong laboratory support for the prevention and control of epidemic situation. At the same time, it took more time for timely treatment of patients’ diseases.