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[目的]研究牛磺酸(Tau)对实验性肝纤维大鼠肝组织的保护作用。[方法]将120只实验用大鼠随机分为正常对照组、模型组、牛磺酸低(150 mg/kg)、中(300 mg/kg)、高(600 mg/kg)剂量组和复方鳖甲软肝片540 mg/kg组,每组20只;采用CCl4复合法制备肝硬化门静脉高压大鼠模型,灌胃给药治疗4周后,测定血清中丙氨酸氨基转移酶门冬氨酸(ALT)、氨基转移酶(AST)和总胆红素(TBIL)含量,测定血清中肝纤四项(CⅣ、PCⅢ、LN、HA)含量,HE染色法观察肝脏组织形态结构变化并对肝组织细胞坏死和组织增生进行分级;通过天狼星染色观察肝脏组织纤维化状况;测定抗氧化酶(SOD、GSH-Px)活性和丙二醛(MDA)含量;测定血清中炎症细胞因子IL-10、TNF-α含量。[结果]与模型组相比,牛磺酸中、高剂量组大鼠血清中ALT、AST、TBIL含量和CⅣ、PCⅢ、HA含量均显著降低(P<0.05,P<0.01),高剂量组LN含量显著降低(P<0.05);牛磺酸各剂量组大鼠肝组织纤维化病变呈不同程度改善,以高剂量组效果最为显著;牛磺酸中、高剂量组大鼠肝脏组织SOD、GSH-Px活性显著升高且MDA含量显著降低(P<0.05,P<0.01),血清中IL-10、TNF-α含量显著降低。[结论]牛磺酸对实验性肝纤维化大鼠具有保护作用,其机制可能与牛磺酸能够有效保肝降酶、抑制氧化应激反应和炎症反应、改善肝功能有关。
[Objective] To study the protective effect of taurine on hepatic tissue in experimental hepatic fibrosis rats. [Methods] A total of 120 experimental rats were randomly divided into normal control group, model group, taurine low (150 mg / kg), medium (300 mg / kg) and high (600 mg / Biejia Ruanganpian 540 mg / kg group, 20 rats in each group. CCl4 compound method was used to prepare cirrhotic portal hypertension rats model. After intragastric administration for 4 weeks, serum alanine aminotransferase aspartate (ALT), total aminotransferase (AST) and total bilirubin (TBIL) were measured. The content of CⅣ, PCⅢ, LN and HA in serum were measured. The morphological changes of liver tissue were observed by HE staining and the Liver tissue necrosis and histopathology were graded. The hepatic fibrosis was observed by Sirius staining. The activities of antioxidant enzymes (SOD, GSH-Px) and malondialdehyde (MDA) were measured. The levels of inflammatory cytokines IL-10 , TNF-α content. [Results] Compared with the model group, the contents of ALT, AST, TBIL and CⅣ, PCⅢ, HA in the medium and high dose taurine groups were significantly decreased (P <0.05, P <0.01) LN significantly decreased (P <0.05). The fibrosis lesions of liver tissue of rats in each dosage group of taurine improved to some extent, especially in high dose group. The contents of SOD, GSH-Px activity was significantly increased and MDA content was significantly decreased (P <0.05, P <0.01), serum IL-10, TNF-α content was significantly reduced. [Conclusion] Taurine has a protective effect on experimental hepatic fibrosis in rats. The mechanism may be related to the fact that taurine can effectively protect the liver and reduce the enzyme, inhibit the oxidative stress reaction and inflammatory reaction, and improve the liver function.