目的:建立高效液相色谱法测定消痔颗粒中黄芩苷的含量。方法:用超声波提取法,采用Spherisorb C18柱,以乙腈-水-醋酸-5％磷酸为流动相,检测波长为278 nm,柱温40℃。结果:平均回收率为101.5％,RSD为0.7％(n=5)。结论:方法简便易行,分离效果好,准确可靠。 Objective: To establish a HPLC method for the determination of baicalin in Xiaoying granules. METHODS: Ultrasonic extraction was performed on a Spherisorb C18 column with acetonitrile-water-acetic acid-5% phosphoric acid as the mobile phase. The detection wavelength was 278 nm and the column temperature was 40°C. Results: The average recovery was 101.5% and the RSD was 0.7% (n=5). Conclusion: The method is simple, easy, accurate, and reliable.