目的探讨骨髓基质干细胞(BMSCs)与支架材料复合,并种植到动物体内后的定位及成骨情况。方法分离培养绿色荧光蛋白(GFP)小鼠BMSCs,体外扩增后与猪脱细胞骨基质材料复合,植入裸鼠背部作为实验组,并以单纯材料植入作为对照组,8周后进行大体观察、常规组织学检查及荧光检测。结果实验组移植物周围部成骨明显,GFP阳性细胞较多;中央部材料降解吸收,GFP阳性细胞少见。对照组成骨较少。结论BMSCs作为种子细胞参与了骨重建,同时GFP示踪是观测BMSCs的一种较好方法。 Objective To investigate the localization and osteogenesis of bone marrow stromal stem cells (BMSCs) and scaffolds after being implanted into animals. Methods Green fluorescent protein (GFP) mouse BMSCs were isolated and cultured, expanded in vitro and combined with porcine acellular bone matrix material. The nude mice were implanted into the back of the mice as the experimental group and the pure materials were implanted as the control group. After 8 weeks, Observation, routine histological examination and fluorescence detection. Results In the experimental group, there was obvious osteogenesis around the graft and more GFP positive cells. The materials in the central part were degraded and absorbed, but GFP positive cells were rare. The control group less bone. Conclusion BMSCs participate in bone remodeling as seed cells, and GFP tracing is a good method to observe BMSCs.