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菜蛾盘绒茧蜂Cotesia vestalis(Haliday)是小菜蛾的重要幼虫内寄生蜂,该蜂在胚胎发育过程中由浆膜产生畸形细胞,随蜂卵孵化,释放到寄主小菜蛾的血腔中。本文运用蛋白质双向电泳技术、电子显微技术和体外培养技术对菜蛾盘绒茧蜂畸形细胞的蛋白质的合成和分泌以及细胞超微形态结构的变化进行了研究。蛋白质双向电泳图谱显示,该细胞内蛋白合成种类极为丰富,尤以分子量40.98～94.64kDa的蛋白种类最多。但在寄生后期,畸形细胞的合成能力下降。超微形态结构发生显著变化表现在胞内细胞器数量减少,出现大量空腔,细胞表面微绒毛联合、变大,内容物外倾。随着蜂幼虫的啮出,有些细胞经历分解过程。体外培养证实,成熟畸形细胞可向培养介质中释放脂溶性物质。此外,在不同饲养温度条件下,畸形细胞伴随蜂幼虫的发育,表现为随温度升高,发育加快的趋势,表明畸形细胞的生长趋势与寄生蜂幼虫发育具有同步性。
The Cotesia vestalis (Haliday), a major larvae of the diamondback moth, is an obligate parasitoid. The bee produces abnormal cells from the serosa during embryonic development and hatches with the egg before it is released into the blood cavity of the host diamondback moth. In this paper, protein biosynthesis, secretion and morphological changes of Dendrolimus tabulaeformis were studied by two-dimensional electrophoresis, electron microscopy and in vitro culture. Protein two-dimensional electrophoresis showed that the cell protein synthesis is extremely rich in species, especially the molecular weight of 40.98 ~ 94.64kDa protein species most. However, in late parasites, deformability of the synthesis of cells decreased. Significant changes in ultrastructural changes in the performance of the number of intracellular organelles decreased, there were a large number of cavities, cell surface microvilli joint, larger, the content of the camber. With bee larvae hitting out, some cells undergo decomposition. In vitro culture confirmed that mature deformable cells can release fat-soluble substances into the culture medium. In addition, under the different feeding temperature, the development of deformity cells accompanied by bee larvae showed the trend of accelerating with the increase of temperature, indicating that the growth trend of deformity cells is synchronous with the development of parasitoid larvae.