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目的探讨低氧诱导因子-1α(HIF-1α)、肝细胞生长因子(HGF)在非小细胞肺癌(NSCLC)中的共表达及其与淋巴管生成的关系。方法应用免疫组织化学方法标记淋巴管,计数微淋巴管密度(LMVD),检测126例NSCLC患者及20例癌旁对照肺组织中HIF-1α、HGF的表达,分析其表达情况与LMVD及临床病理参数之间的关系。结果 HIF-1α与HGF的表达呈明显相关(R2=0.590,P<0.05);NSCLC组织中存在HIF-1α和HGF的高表达,阳性表达率分别为85.7%和80.9%,明显高于癌旁对照肺组织(P均<0.05),癌组织中的LMVD明显高于癌旁对照肺组织(17.525 0±2.286 9 vs 1.765 0±0.735 3,P<0.05);HIF-1α或HGF阳性表达的NSCLC患者较阴性者有更高的LMVD(P<0.05),HIF-1α、HGF的阳性表达与TNM分期、淋巴结转移显著相关。结论在NSCLC中存在HIF-1α与HGF的共表达,该表达可促进NSCLC淋巴管的新生及淋巴转移。
Objective To investigate the expression of HIF-1α and hepatocellular growth factor (HGF) in non-small cell lung cancer (NSCLC) and their relationship with lymphangiogenesis. Methods The lymphatic vessels were labeled by immunohistochemistry and the lymphatic vessel density (LMVD) was counted. The expressions of HIF-1α and HGF in 126 NSCLC patients and 20 adjacent non-cancerous lung tissues were detected. The expression of HIF-1α, The relationship between parameters. Results The expression of HIF-1α and HGF was significantly correlated (R2 = 0.590, P <0.05). The positive expression rates of HIF-1α and HGF in NSCLC tissues were 85.7% and 80.9% (P <0.05). The LMVD in cancerous tissues was significantly higher than that in adjacent non-cancerous lung tissues (17.525 ± 2.286 9 vs 1.765 ± 0.735 3, P <0.05). The expression of HIF-1α or HGF positive NSCLC Patients with negative expression had higher LMVD (P <0.05). The positive expressions of HIF-1α and HGF were significantly associated with TNM stage and lymph node metastasis. Conclusions The co-expression of HIF-1α and HGF exists in NSCLC, which may promote lymphangiogenesis and lymph node metastasis in NSCLC.