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目的观察六味地黄汤对单侧输尿管梗阻(UUO)大鼠模型中胶原IV(ColIV)及基质金属蛋白酶抑制物-1(TIMP-1)表达的影响。方法将输尿管梗阻大鼠随机分为模型组,依那普利组,六味地黄汤组,假手术组,14d后采用免疫组织化学检测ColIV和TIMP-1的表达水平。结果与假手术组比较,其余3组ColIV和TIMP-1表达均明显高于假手术组(P<0.01),六味地黄汤组ColIV和TIMP-1表达明显低于模型组(P<0.01),高于依那普利组(P<0.05)。结论六味地黄汤可下调TIMP-1和ColIV的表达,减缓UUO大鼠肾小管间质损害,可作为梗阻性肾病辅助用药。
Objective To observe the effect of Liuwei Dihuang Decoction on the expression of collagen IV (Col IV) and matrix metalloproteinase inhibitor-1 (TIMP-1) in unilateral ureteral obstruction (UUO) rat model. Methods Rats with ureteral obstruction were randomly divided into model group, enalapril group, Liuwei Dihuang Decoction group and sham operation group. The expression of Col IV and TIMP-1 was detected by immunohistochemistry 14 days later. Results Compared with the sham group, the expression of ColIV and TIMP-1 in the other 3 groups was significantly higher than that in the sham group (P<0.01). The expression of ColIV and TIMP-1 in Liuwei Dihuang Decoction group was significantly lower than that in the model group (P<0.01). Higher than enalapril group (P<0.05). Conclusion Liuwei Dihuang Decoction can down-regulate the expression of TIMP-1 and Col IV, slow down the damage of renal tubulointerstitium in UUO rats, and can be used as an adjuvant drug for obstructive nephropathy.