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目的探讨高糖饮食对幼鼠学习记忆能力和情绪的影响,适量补充钙剂是否产生改善作用。方法以幼鼠(生后30~35d)为研究对象,给予连续3周高糖饮食后,应用Morris水迷宫检测幼鼠学习记忆能力,强迫游泳检测抑郁行为,高架十字迷宫检测焦虑行为,并观察高糖饮食并补充钙剂后动物血清钙含量的变化及其与上述指标的相关性。结果连续高糖饮食后,幼鼠在Morris水迷宫四个象限的逃避潜伏期均明显延长[(81.2±7.9)s,(76.8±8.4)s,(67.4±6.9)s和(61.3±6.4)s],而穿越站台次数(1.4±0.6)较正常组(3.8±0.9)减少,而钙剂补充对此并未产生明显的改善作用;强迫游泳实验的漂浮潜伏期[(99.5±10.8)s]缩短,而漂浮时间[(432.8±11.2)s]显著延长,适量补充钙剂使这些指标[(184.5±10.1)s和(332.2±12.3)s)趋于正常;而高架十字迷宫实验的各项指标在高糖饮食组和补充钙剂组均未出现明显异常。结论高糖饮食能够造成幼鼠学习记忆能力的减退和抑郁表现,适量补充钙剂可以改善抑郁行为,但无助于学习记忆能力的恢复。
Objective To investigate the effect of high-sugar diet on learning and memory abilities and emotion in young rats, and to find out whether the supplement of calcium can improve the body weight. Methods The pups (30-35 days after birth) were enrolled in this study. After 3 consecutive weeks of high-carbohydrate diet, Morris water maze test was used to assess the learning and memory ability, forced swimming test for depression and elevated maze test for anxiety. The changes of serum calcium in high glucose diet and calcium supplementation and its correlation with the above indexes. Results After continuous high-glucose diet, the escape latency in the four quadrants of the Morris water maze was significantly longer ([81.2 ± 7.9] s, (76.8 ± 8.4) s, (67.4 ± 6.9) s and [61.3 ± 6.4] s (1.4 ± 0.6) decreased compared with that of the normal group (3.8 ± 0.9), while calcium supplementation had no significant improvement. The forced incubation period (99.5 ± 10.8 s s) was shortened , While the floating time [(432.8 ± 11.2) s] was significantly prolonged. Appropriate amounts of calcium supplementation made these indexes [(184.5 ± 10.1) s and (332.2 ± 12.3) s) No obvious abnormalities were found in the high-sugar diet group and the calcium supplement group. Conclusions High-sugar diet can reduce learning and memory ability and depression in young rats. Appropriate amount of calcium supplementation can improve depression, but does not help the recovery of learning and memory ability.