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用Ames方法测定了硒和硒酵母对MNNG诱发TA100致突变作用的抑制效果,并用原子吸收分光光度计测定12例浅表性胃炎(SG)、17例萎缩性胃炎(CAG)、6例不典型增生(Dys)和12例胃癌(GC)病人的头发、血液和部分患者胃液中的硒含量,并用气相-热能分析仪测定了胃液中的亚硝胺含量。结果表明:硒和硒酵母有抑制MNNG对TA100的致突变作用,抑制率分别为66.5％和37.9％;患者的血清硒、发硒和胃液硒的含量随胃粘膜病变程度的加重而降低;SG与GC之间差异非常显著(p<0.01),而亚硝胺的含量SG明显低于CAG、Dys及GC。应用硒酵母给予患者补充硒(150μg/d),观察到血硒含量有明显提高,MnSOD及LPO下降,NK细胞活性增加和胃镜病理活检有好转的变化。
Ames method was used to determine the inhibitory effect of selenium and selenium yeast on MNNG-induced TA100-induced mutagenesis, and 12 cases of superficial gastritis (SG), 17 cases of atrophic gastritis (CAG) and 6 cases of atypical pneumonia were detected by atomic absorption spectrophotometer (Dys) and 12 cases of gastric cancer (GC) patients with hair, blood and gastric juice in some patients with selenium content, and gas phase - thermal analyzer nitrosamine content in gastric juice was measured. The results showed that selenium and selenium yeast could inhibit the mutagenicity of MNNG to TA100 with the inhibitory rates of 66.5% and 37.9%, respectively. The contents of serum selenium, selenium and gastric selenium decreased with the severity of gastric mucosal lesion. SG And GC (p <0.01). However, the content of nitrosamines SG was significantly lower than that of CAG, Dys and GC. Application of selenium yeast to patients with selenium (150μg / d), blood selenium levels were significantly increased, decreased MnSOD and LPO, NK cell activity and endoscopic biopsy improved.