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目的 通过高效液相色谱法测定CTX免疫抑制小鼠模型中CTX的血药代谢浓度 ,观察CTX对小鼠免疫功能的影响 ,来评价CTX免疫抑制小鼠模型用于SRCA的可行性及实用性。方法 (1)CTX 0 .2g/kg ,行昆明小鼠皮下注射 ,制作CTX免疫抑制小鼠模型。 (2 )高效液相色谱法测定经CTX处理之后的小鼠2 4、4 8h血药浓度。 (3)测定经CTX处理后 2、4、6、8、10d小鼠免疫功能变化。结果 (1)予CTX后 2 4h 4只小鼠的平均血药浓度为 15 2± 95 μg/L ;予CTX后 4 8h另 4次小鼠的平均血药浓度为 2± 2 μg/L。 (2 )第 2、4、6d实验组和对照组的白细胞、淋巴细胞均有显著性差异。结论 CTX免疫抑制小鼠经 4 8h体内代谢之后 ,CTX基本上消除 ,此时行SRCA ,CTX不会对移植瘤起抗瘤效应。 6天法CTX免疫抑制小鼠模型能替代裸鼠
Objective To determine the metabolic concentration of CTX in CTX immunosuppressive mouse model by high performance liquid chromatography (HPLC) and observe the effect of CTX on immune function of mice to evaluate the feasibility and practicality of CTX immunosuppressive mouse model for SRCA. Methods (1)CTX 0.2g/kg was subcutaneously injected into Kunming mice to establish a CTX immunosuppressive mouse model. (2) High-performance liquid chromatography was used to measure the plasma concentrations of mice after CTX treatment for 24 and 48 hours. (3) Determine the immune function changes in mice 2, 4, 6, 8, and 10 days after CTX treatment. Results (1) The mean plasma concentration of 4 mice was 152±95 μg/L at 24 h after CTX administration; the average plasma concentration of mice was 2±2 μg/L at 4 h after CTX administration. (2) There were significant differences in leukocytes and lymphocytes between the 2nd, 4th, and 6th days in the experimental group and the control group. Conclusion After CTX immunosuppressed mice were metabolized in vivo for 48 hours, CTX was basically eliminated. At this time, SRCA was performed. CTX did not have anti-tumor effect on transplanted tumors. 6-day CTX immunosuppressive mouse model can replace nude mice