论文部分内容阅读
目的 :探讨门静脉内注射供体脾细胞诱导移植肾的免疫耐受情况。方法 :实验组 Wistar大鼠在肾移植同时将经过预处理的供体 SD大鼠脾细胞注入门静脉 ,对照组则注入生理盐水 ,然后用环孢素 A治疗 1周 ,并以大鼠平均存活时间为标准比较两组结果。结果 :对照组平均存活( 1 0 .5± 2 .1 ) d,实验组为 ( 72 .2± 32 .0 ) d( P <0 .0 1 )。实验组在肾移植 60 d后 ,再移植 SD大鼠和Lewis大鼠的皮肤 ,发现 SD大鼠的皮肤不被排异 ,Lewis大鼠的皮肤出现排异。结论 :门静脉内注射供体脾细胞可诱导肾移植免疫耐受 ,并且这种耐受具有特异性。
Objective: To investigate the immune tolerance of transplanted kidneys induced by portal vein injection of donor spleen cells. Methods: Wistar rats in experimental group were injected with splenocytes of donor SD rat spleen into the portal vein while renal transplantation. The control group was injected with normal saline and then treated with cyclosporin A for 1 week. The mean survival time As a standard comparison of two groups of results. Results: The control group survived on average (105 ± 2.1 days) and the experimental group (72.2 ± 32.0 days) (P <0.01). In the experimental group, the skin of SD rats and Lewis rats was transplanted 60 days after kidney transplantation, and the skin of SD rats was not differentiated. The appearance of Lewis rats’ skin appeared abnormal. Conclusions: Intravenous injection of donor splenocytes induces immune tolerance in renal transplant recipients, and this tolerance is specific.