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应用ＰＣＲ的方法，从人的染色体片段制备人α_２ｂ干扰素的ｃＤＮＡ，将其克隆到ｐＲＣ_（２３）表达载体上，构建成重组表达质粒ｐＭＺＩ_２Ａ和ｐＭＺＩ_２Ｂ，并转化大肠杆菌ＤＨ_（５α）组建成工程菌。ｐＭＺＩ_２Ａ和ｐＭＺＩ_２Ｂ的区别在于后者的α_２ｂ基因５＇－端起始密码ＡＴＧ上游编制了一段ＳＤ顺序，与ｐＬ启动子上连接的ＳＤ顺序组成双ＳＤ顺序。表达重组质粒中由于不含ｃＩｔＳ基因，所以采取３７℃连续培养后比较两者的抗病毒活性，表明ｐＭＺＩ_２Ｂ的表达比ｐＭＺＩ_２Ａ有明显的提高，分别为１．６９×１０￣４ＩＵ／ｍｌ和３．４６×１０￣３ＩＵ／ｍｌ。
The cDNA of human α2b interferon was prepared from human chromosome fragment by PCR and cloned into pRC_ (23) expression vector. The recombinant plasmid pMZI_2A and pMZI_2B were constructed and transformed into E. coli DH_ (5α) bacteria. The difference between pMZI_2A and pMZI_2B is that an SD sequence is made upstream of the 5’-end start codon of the α_2b gene of the latter α-2b gene and a double-sequence is formed with the SD sequence linked to the pL promoter. The expression of pMZI_2B in pMZI_2A was significantly higher than that in pMZI_2A, which was 1.69 × 10 ~ 4IU / ml and 3, respectively, since cItS gene was not expressed in the recombinant plasmid. 46 × 10 ~ 3IU / ml.