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IF 有抑制病毒复制、抑制细胞增殖、调节免疫反应、抗肿瘤等作用。本文报告,用以治疗人体肿瘤的那批人白细胞干扰素制品,能增强正常人非致敏淋巴细胞体外杀伤同种异体肿瘤细胞的胞毒作用。所用的靶细胞系为:从 Burkitt 淋巴瘤来的Raji 细胞,从人羊水细胞来的 U 细胞,从骨肉瘤来的393T 细胞及2T 细胞,从人肝来的张细胞。用[~3H]胸苷法测定 DNA 的合成。用微池法及(~51)Cr 释出法测定胞毒作用。测定的结果是:1.IF 及正常人淋巴细胞分别能抑制 Raji 细胞的 DNA 合成,但二者同时使用效果较为明显。这种协同作用可能是由于 IF 对淋巴
IF inhibit viral replication, inhibition of cell proliferation, regulating immune response, anti-tumor effect. This article reports that those human leukocyte interferon products that are used to treat human tumors can enhance the cytotoxicity of normal human non-sensitized lymphocytes to kill allogeneic tumor cells in vitro. The target cell lines used were Raji cells from Burkitt’s lymphoma, U cells from human amniotic fluid, 393T cells from osteosarcoma and 2T cells, and from human liver. DNA synthesis was measured by [~ 3H] thymidine method. Cytotoxicity was determined by the microcell method and (~ 51) Cr release method. The results are as follows: 1.IF and normal lymphocytes can inhibit DNA synthesis of Raji cells, respectively, but at the same time the effect of the two is more obvious. This synergy may be due to IF on lymph