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聚丙烯酰胺凝胶电泳具有设备简单、操作简便、分辨力高等优点,日益被人们广泛应用。为了进一步提高分辨力,在聚丙烯酰胺凝胶电泳的基础上又发展了凝胶浓度梯度电泳、双向电泳,及其与等电点聚焦电泳、免疫电泳等结合使用的电泳分析方法。为开展分子生物学、细胞生物学以及免疫学等方面的研究,甚至临床诊断研究,提供了—个有效的手段。聚丙烯酰胺凝胶—琼脂糖凝胶交叉免疫电泳是一种抗原先在聚丙烯酰胺凝胶中电泳,接着在与第一向电泳成垂直方向的含抗体的琼脂糖凝胶上走第二向电泳的方法,在电场作用下,聚丙烯酰胺凝胶电泳分离后的抗原又进入含有抗体的琼脂糖凝胶中,抗体也同时泳动,但由于抗体是γ-球蛋白和电
Polyacrylamide gel electrophoresis has the advantages of simple equipment, easy operation and high resolution, and is widely used by people. In order to further improve the resolution, based on the polyacrylamide gel electrophoresis and the development of gel concentration gradient electrophoresis, two-dimensional electrophoresis, and its isoelectric focusing with electrophoresis, immunoelectrophoresis and other methods of electrophoresis analysis. To carry out molecular biology, cell biology and immunology research, and even clinical diagnosis research, provides an effective means. Polyacrylamide Gel-Sepharose Cross-Immuno-Electrophoresis is an antigen that has been electrophoresed on a polyacrylamide gel first, followed by a second step on an antibody-containing agarose gel oriented perpendicular to the first one Electrophoresis method, under the action of an electric field, the polyacrylamide gel electrophoresis of the antigen and then separated into the antibody-containing agarose gel, the antibody also moves at the same time, but the antibody is gamma-globulin and electricity