在正常的养殖条件下,使试验水体的氨浓度逐步升高,从而造成牛蛙慢性中毒,牛蛙慢性中毒昏迷或死亡后,解剖并取其内脏组织如肝脏、脾脏、胸腺、肠、肾脏、血液等制作病理切片或涂片。观察比较,发现中毒后的牛蛙胸腺、肝脏明显肿大,胸腺颜色变为淡黄色,脾脏表面变得粗糙等。显微镜检,细胞出现肿大、空泡化、坏死等一系列组织病理学变化。 Under normal farming conditions, the concentration of ammonia in the test water gradually increases, resulting in chronic poisoning of bullfrogs, chronic poisoning of bullfrogs, or death and dissection and removal of visceral tissues such as liver, spleen, thymus, intestine, kidney, blood, etc. Make pathological sections or smears. Observation and comparison, found that poisoned bullfrog thymus, the liver was significantly enlarged, the color of the thymus turned pale yellow, the surface of the spleen becomes rough and so on. Microscopy, cell swelling, vacuolization, necrosis and a series of histopathological changes.