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1973—2006年对我国东北山葡萄抗寒的种质资源(品种、品系)与不抗寒的欧亚种酿造葡萄品种进行种间杂交、回交和重复杂交。共定植42个组合杂种苗,定植成活7680株。观察42个组合7680株杂种苗F1￣F4代对霜霉病抗性的的抗性分离,共分离出抗病(2￣3级)3747株,占杂种苗总株数48.8%。其中抗病×抗病的10个组合共分离出抗病2303株、占69.3%,分别高于抗病×不抗病、中等感病×中等感病、中等感病×高感病、高感病×高感病的组合的23.2、29.2、45.3和54.8个百分点。遗传规律是:山葡萄种间杂交后代F1￣F4对霜霉病抗性分离,表现为连续分布,倾向于抗病亲本,后代群体抗病性主要由亲本抗病性决定,杂交组合中抗病亲本越多,分离出的抗病单株越多,为多基因控制的数量性状遗传、“抗病基因”有累加效应、呈显性遗传给后代。已选育出抗霜霉病、抗寒、可酿造干红山葡萄酒的新品种左优红、品系94-7-75、94-8-168、95-2-482和2002-1-135。
From 1973 to 2006, interspecific hybridization, backcrossing and repeated crosses were carried out on cold hardy germplasm resources (varieties and lines) of northeastern mountain grape in China and those of European and Asian grape cultivars not resistant to cold. Co-planting 42 combinations of hybrid seedlings, planting 7680 survival. A total of 3747 resistant strains (2 ~ 3 grade) were isolated from F1 ~ F4 hybrids of 7680 hybrid seedlings from 42 combinations, 48.7% of the total number of hybrid seedlings were observed. Among them, 2303 strains were resistant to disease and resistant to disease, accounting for 69.3% of the total, respectively, which were higher than that of resistant disease × non-resistant disease, moderately susceptible disease × moderately-senile disease, moderately-senile disease × highly-susceptible and highly-susceptible 23.2, 29.2, 45.3 and 54.8 percentage points of the combination of the disease x high susceptible. The genetic rule is that F1 ~ F4 segregation of resistance to downy mildew in F1 hybrids between F1 and F2 hybrids was observed in continuous distribution and tended to be the parents of disease-resistant parents. The disease resistance of offspring population was mainly determined by disease resistance of parents, The more disease-resistant individuals isolated, the more inherited the quantitative traits controlled by multiple genes. The “disease-resistance genes” have additive effects and are dominantly inherited to offspring. Has developed a new anti-downy mildew resistance, cold, dry red wine can be red Zuo Youhong, strains 94-7-75,94-8-168,95-2-482 and 2002-1-135.