论文部分内容阅读
丙型肝炎是由丙型肝炎病毒(hepatitis C virus,HCV)感染所导致的传染性肝病,呈现世界性流行态势,严重危害人类健康。由于病毒自身高度突变,以及广泛高效的细胞培养体系和合适的小动物模型缺乏,目前尚无可有效预防的疫苗。自1989年丙型肝炎病毒基因组首次被确定以来,Con1(1b)亚基因组复制子和JFH1(2a)毒株细胞培养体系相继建立。以此为工具,HCV生活周期多个关键环节得以阐明。近年来,研究者在Con1亚基因组复制子、JFH1和J6/JFH1细胞培养体系的基础上,构建出多个基因型和亚型的复制子和细胞培养体系。不同的体系在HCV复制与致病机制研究、抗病毒药物筛选方面,具有不同的用途及优缺点。针对HCV复制子与细胞培养体系的研究进展进行综述,可为HCV的相关研究提供参考。
Hepatitis C is an infectious liver disease caused by the infection of hepatitis C virus (HCV), presenting a worldwide epidemic that seriously endangers human health. Due to the highly mutated virus itself, as well as the extensive and efficient cell culture system and the absence of suitable small animal models, no vaccine is currently available for effective prevention. Since the hepatitis C virus genome was first identified in 1989, the subculture of the Con1 (1b) subgenomic replicon and the JFH1 (2a) strain cell culture system were established. Using this as a tool, several key aspects of the HCV life cycle are elucidated. In recent years, researchers have constructed a number of genotypes and subtypes of replicons and cell culture systems based on the Con1 subgenomic replicon, JFH1 and J6 / JFH1 cell culture systems. Different systems have different uses, advantages and disadvantages in the study of HCV replication and pathogenesis, antiviral drug screening. The review on the research progress of HCV replicon and cell culture system can provide reference for the related research of HCV.