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目的观察帕金森病模型大鼠纹状体内去甲肾上腺素(NA)、5-羟色胺(5-HT)的含量以及针刀干预后的变化,探讨针刀治疗帕金森病的作用机制。方法成年健康雄性SD大鼠76只,随机分为2组,实验组60只,空白组16只。实验组60只采用右侧纹状体双靶点注射6-羟多巴胺(6-OHDA)造模,造模结束后用阿朴吗啡(APO)诱导法进行模型评价,并通过行为学检测,实验组共有48只大鼠造模成功,随机分为模型组、药物组和针刀组,每组16只。空白组和模型组正常抓取刺激,不进行干预治疗;药物组予美多芭片悬浊液治疗,每日1次,连续4周;针刀组进行松解干预,每周2次,连续4周。治疗结束1周后进行行为学评价,通过苏木精-伊红(HE)染色图片观察大鼠纹状体的病理学变化,通过高效液相色谱法观察大鼠纹状体NA、5-HT的含量。结果行为学评价结果显示,空白组大鼠未见旋转,模型组大鼠出现旋转,干预后药物组及针刀组大鼠旋转圈数明显减少(P<0.01),且治疗前后比较差异有统计学意义(P<0.01)。与空白组比较,模型组大鼠纹状体内NA、5-HT含量减少(P<0.05);与模型组比较,药物组和针刀组大鼠纹状体中NA、5-HT含量显著增高(P<0.05)。结论针刀对帕金森病模型大鼠的治疗作用可能与其松解颈部肌肉,改善局部血液循环,增加NA、5-HT的含量,从而减轻帕金森病的症状有关。
Objective To investigate the changes of noradrenaline (NA) and 5-hydroxytryptamine (5-HT) in the striatum of Parkinson’s disease model rats and the changes after acupuncture intervention, and to explore the mechanism of acupotome treatment of Parkinson’s disease. Methods Sixty-six healthy adult male Sprague-Dawley rats were randomly divided into two groups, 60 in experimental group and 16 in blank group. 60 rabbits in the experimental group were injected with 6-OHDA (6-hydroxydopamine) on the right striatum. After the model was established, APO induction was used to evaluate the model. The behavioral tests, A total of 48 rats were randomly divided into model group, drug group and acupotome group, 16 rats in each group. The blank group and the model group were grasped and stimulated normally without intervening treatment. The drug group was treated with Mendoba Tablets once a day for 4 weeks, and the acupotome group was subjected to loosening intervention twice a week 4 weeks. Behavioral evaluation was performed one week after the end of treatment. Pathological changes of striatum were observed by hematoxylin-eosin (HE) staining and pathological changes of rat striatum NA and 5-HT were observed by high performance liquid chromatography Content. Results The results of behavioral evaluation showed that the rats in the blank group did not rotate, the rats in the model group appeared to rotate, and the numbers of rotations of the rats in the drug-treated group and the acupotome group were significantly decreased (P <0.01) after intervention, with statistical differences before and after treatment Significance (P <0.01). Compared with the blank group, the content of NA and 5-HT in the striatum of the model group decreased (P <0.05). Compared with the model group, the contents of NA and 5-HT in the striatum of the drug group and the acupotome group were significantly increased (P <0.05). Conclusion The therapeutic effect of acupotomy on rats with Parkinson’s disease may be related to the release of the neck muscles, the improvement of local blood circulation, the increase of NA, 5-HT content and the alleviation of the symptoms of Parkinson’s disease.