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利用蛋白质复性电泳技术,研究了低能N+注入介导玉米DNA的水稻幼苗叶片和根系中性蛋白水解酶的表达。用能量为25 keV、剂量为3.0×1017N+/cm2的氮离子束对水稻豫粳6号种胚进行照射处理,照射的种子于质量浓度为100μg/mL玉米基因组DNA介导液中进行转化处理,其他处理同时进行,结果表明,各处理均引起水稻幼苗叶片和根系中性蛋白水解酶种类及活性差异表达,叶片与根系中均检测出多条差异酶带,离子束介导玉米DNA的处理中性蛋白水解酶带变化活跃,有新酶带表达,也有部分酶带缺失。说明低能离子束介导远缘大分子DNA引起水稻幼苗蛋白质表达的差异。
Protein neutral electrophoresis was used to study the expression of neutral protease in leaves and roots of rice seedlings mediated by low energy N +. The rice embryos of Yujing 6 were irradiated with 25 keV and 3.0 × 10 17 N + / cm 2 doses of nitrogen ion beam. The irradiated seeds were transformed in the medium of 100 μg / mL maize genomic DNA. Other treatments were carried out simultaneously. The results showed that all the treatments resulted in the differential expression of the types and activities of neutral proteolytic enzymes in leaves and roots of rice seedlings, and many different bands were detected in leaves and roots. Ion beam-mediated maize DNA processing Proteolytic bands with changes in the active, a new enzyme band expression, but also some lack of enzyme band. Indicating that low energy ion beam mediated the distant macromolecular DNA caused by rice seedlings protein expression differences.