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目的:研究γ-干扰素(γ-IFN)对他莫昔芬(TAM)抗乳腺癌细胞的增强作用。方法:在体外培养条件下,分别或联合应用γ-干扰素、TAM或雌二醇(E2)作用于雌激素受体(estrogen receptor,ER)阳性的MCF-7和ER阴性的MDA-MBa-231人乳腺癌细胞株,用MT比色法分析细胞生长抑制作用,流式细胞仪检测细胞周期分布,DNA凝胶电泳和流式细胞仪检测细胞凋亡。结果:TAM能抑制ER阳性和阴性的乳腺癌细胞的生长,阻滞细胞周期于G0／G1期,并可诱导细胞凋亡;相同浓度条件下,TAM对ER阳性乳腺癌细胞的抑制作用强于对ER阴性的乳腺癌细胞。同时,TAM能拮抗外源性雌激素对MCF-7细胞的促生长作用,而对MDA-MB-231细胞的生长抑制作用与雌激素的存在与否无关。γ-干扰素预处理细胞24 h后,TAM抗乳腺癌细胞的作用增强。结论:体外条件下,TAM有抗ER阳性和阴性乳腺癌细胞作用,作用机制是通过影响细胞周期,诱导细胞凋亡,γ-干扰素能加强TAM的抗乳腺癌作用。
OBJECTIVE: To study the effect of γ-interferon (γ-IFN) on the enhancement of tamoxifen (TAM) against breast cancer cells. METHODS: Under the in vitro culture conditions, γ-interferon, TAM or estradiol (E2) was used in combination with oestrogen receptor (ER)-positive MCF-7 and ER-negative MDA-MBa- In 231 human breast cancer cell lines, cell growth inhibition was analyzed by MT colorimetry, cell cycle distribution was measured by flow cytometry, DNA gel electrophoresis and flow cytometry were used to detect cell apoptosis. RESULTS: TAM inhibited the growth of ER positive and negative breast cancer cells, arrested cell cycle at G0/G1 phase, and induced apoptosis; at the same concentration, TAM inhibited ER positive breast cancer cells more strongly than TAM. For ER-negative breast cancer cells. At the same time, TAM can antagonize the growth-promoting effect of exogenous estrogen on MCF-7 cells, but the growth inhibition of MDA-MB-231 cells has nothing to do with the presence or absence of estrogen. After pretreatment of cells with γ-interferon for 24 h, the effect of TAM against breast cancer cells was enhanced. Conclusion: Under in vitro conditions, TAM has anti-ER positive and negative breast cancer cells, the mechanism of action is to affect cell cycle, induce apoptosis, γ-interferon can enhance the role of TAM anti-breast cancer.