论文部分内容阅读
目的研究富含亮氨酸重复单位的G蛋白偶联受体5(Lgr5)和巨噬细胞游走抑制因子(MIF)在结直肠癌中的表达及其与临床病理因素之间的相关性。方法收集2014年5月至2016年5月结直肠癌石蜡包埋标本64例。采用免疫组化检测34例正常肠道组织、40例肠上皮内瘤变、64例结直肠癌组织中的Lgr5和MIF的表达,并分析其与临床病理因素之间的关系。结果 Lgr5在正常肠道组织、肠上皮内瘤变以及结直肠癌中的阳性表达率分别为6.88%、47.50%、84.38%,呈逐渐增高的趋势,且两两比较差异均有统计学意义(P均<0.01)。MIF在正常组织、肠上皮内瘤变以及结直肠癌组织中的表达率分别为17.65%、40.00%、81.25%,也呈逐渐增高的趋势,且两两比较差异均有统计学意义(P均<0.01)。结直肠癌组织中的Lgr5、MIF高表达与病理分级、Dukes分期以及有无淋巴结转移等临床病理因素有关(P<0.05,P<0.01),而与性别、年龄无关(P均>0.05)。结论 Lgr5和MIF在结直肠癌组织中呈高表达,且与其发生、发展、侵袭、转移密切相关,对上述因子的调控有可能成为结直肠癌治疗的新的靶点。
Objective To investigate the expression of Lgr5 and MIF in colorectal cancer and its correlation with clinical pathological factors. Methods Sixty-four paraffin-embedded specimens of colorectal cancer from May 2014 to May 2016 were collected. Immunohistochemistry was used to detect the expression of Lgr5 and MIF in 34 cases of normal intestinal tissue, 40 cases of intestinal intraepithelial neoplasia and 64 cases of colorectal cancer. The relationship between Lgr5 and MIF was analyzed. Results The positive rates of Lgr5 in normal intestinal tissue, intestinal intraepithelial neoplasia and colorectal cancer were 6.88%, 47.50% and 84.38%, respectively. The positive rates of Lgr5 were gradually increased, and the differences were statistically significant (P < P <0.01). The expression rates of MIF in normal tissue, intestinal intraepithelial neoplasia and colorectal cancer tissues were 17.65%, 40.00% and 81.25%, respectively, which showed a trend of increasing gradually with a significant difference (P < <0.01). The high expressions of Lgr5 and MIF in colorectal cancer were correlated with clinicopathologic factors such as pathological grade, Dukes staging and lymph node metastasis (P <0.05, P <0.01), but not gender and age (all P> 0.05). Conclusion Lgr5 and MIF are highly expressed in colorectal cancer tissues, and are closely related to their occurrence, development, invasion and metastasis. The regulation of these factors may become a new target for the treatment of colorectal cancer.