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目的 :建立测定尿中的香草扁桃酸 (VMA)含量的高效液相色谱法。方法 :以Nova PakC18柱为分析柱 ,流速是 0 9ml·min-1,流动相为 40mmol·L-1NaH2 PO4 液 (内含Na2 EDTA和辛基磺酸钠各为 5 0mg·L-1,pH3.0 ) ,采用电化学检测器和利用内标法测定了 30例正常人 2 4h尿中的VMA含量。结果 :当VMA浓度为 5～ 2 0 0 μmol·L-1时呈良好的线性关系。VMA的平均回收率为 99 1% ,平均批内变异系数为 5 8% ,平均批间变异系数为 7 7% ,最低检出量是 5 3 2μg。标本量为 10 0 μl时的最低检出浓度为 0 2 8μmol·L-1;正常人 2 4h尿中VMA含量为 (18 13± 6 17) μmol。结论 :该方法能准确测定尿中VMA含量 ,适合临床常规应用。
Objective : To establish a HPLC method for the determination of vanillyl mandelic acid (VMA) in urine. Methods: A Nova Pak C18 column was used as the analytical column. The flow rate was 0 9ml·min-1. The mobile phase was 40mmol·L-1NaH2PO4 solution (containing Na2 EDTA and sodium octyl sulfonate were 50mg·L-1, pH3 respectively. .0) Using an electrochemical detector and using an internal standard method to measure the content of VMA in 24 urine of 30 normal subjects. RESULTS: A good linear relationship was observed when the concentration of VMA was 5 to 200 μmol·L-1. The average recovery rate of VMA was 99 1%, the mean intra-assay coefficient of variation was 58%, the average inter-assay coefficient of variation was 77%, and the minimum detectable amount was 532μg. The minimum detectable concentration was 0 28 μmol·L-1 when the volume of specimen was 100 μl, and the content of VMA was (18 13±6 17) μmol in 24 hours. Conclusion : This method can accurately determine the content of VMA in urine and is suitable for clinical routine application.