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目的上皮-间充质转化(EMT)能诱导CD44~+CD24~(-low)乳腺癌干细胞的产生,白细胞介素-6(IL-6)是很强的EMT诱导因子,探讨IL-6是否通过诱导CD44~+CD24~(-low)细胞的产生,从而促进乳腺癌MCF7细胞的辐射抵抗。方法含50 ng/ml IL-6的培养基培养MCF7细胞,流式细胞术检测CD44~+CD24~(-low)乳腺癌干细胞标记的变化,实时定量聚合酶链反应(Real-time PCR)及Western blot检测EMT标记分子的表达水平,进一步利用流式细胞术从诱导组中分选出CD44~+CD24~(-low)细胞,克隆形成实验检测分选后细胞的辐射抵抗能力。结果 IL-6能诱导MCF7细胞发生间充质样转化,诱导5 d后CD44~+CD24~(-low)细胞显著富集,诱导形成的CD44~+CD24~(-low)细胞的辐射抵抗能力显著提高。结论 IL-6诱导了CD44~+CD24~(-low)乳腺癌干细胞的产生,从而促进MCF7细胞的辐射抵抗。
Objective Epithelial-mesenchymal transition (EMT) induces the production of CD44 ~ + CD24 ~ (-low) breast cancer stem cells, and interleukin-6 (IL-6) is a strong EMT inducing factor. By inducing the production of CD44 ~ + CD24 ~ (-low) cells, thereby promoting the radiation resistance of breast cancer MCF7 cells. Methods MCF7 cells were cultured in a medium containing 50 ng / ml IL-6. Flow cytometry was used to detect the changes of CD44 + CD24 ~ -low breast cancer stem cell markers. Real-time PCR and real- Western blot was used to detect the expression of EMT marker molecules. CD44 ~ + CD24 ~ (-low) cells were further sorted out from the induction group by flow cytometry. The colony formation assay was used to detect the radiation resistance of the cells. Results IL-6 could induce MCF7 cells to undergo mesenchymal transformation and induce CD44 ~ + CD24 ~ (-low) cells to be significantly enriched after 5 days of induction and induce the formation of CD44 ~ + CD24 ~ (-low) cells. Significantly increased. Conclusion IL-6 induces the production of CD44 ~ + CD24 ~ -low breast cancer stem cells and promotes the radiation resistance of MCF7 cells.