论文部分内容阅读
目的研究替米沙坦对非酒精性脂肪肝大鼠炎症反应及肝纤维化的影响。方法雄性SD大鼠以高脂饲料喂养12周造模,正常组(n=10)以普通饲料喂养12周。模型大鼠按照体重随机分为3组:模型组(n=15,1.0 mL·kg~(-1)·d~(-1)生理盐水)、对照组(n=10,8.4 mg·kg~(-1)·d~(-1)多烯磷脂酰胆碱)和实验组(n=10,8 mg·kg~(-1)·d~(-1)替米沙坦),并继续给予高脂饲料喂养。16周后处死大鼠,用全自动生化分析仪检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、三酰甘油(TG)、总胆固醇(TC)、白细胞介素-6(IL-6)、IL-8和肿瘤坏死因子-α(TNF-α)水平及大鼠肝组织性形态学变化。结果给药16周大鼠体重,模型组为(582.63±33.59)g,正常组为(484.82±33.69)g,组间比较差异有统计学意义(P<0.001)。对照组及实验组大鼠体重分别为(538.54±39.46),(532.71±40.85)g,与模型组比较差异有统计学意义(P<0.01)。模型组大鼠肝指数为4.01±0.15,正常组为2.98±0.29,组间比较差异有统计学意义(P<0.00)。对照组的肝指数为3.54±0.12、实验组为3.65±0.13,与模型组比较差异有统计学意义(均P<0.001)。与模型组小叶内炎症为2.80±0.42、NSA评分为7.70±0.48及纤维化11.25±2.12相比,对照组的小叶内炎症1.42±0.71、NSA评分5.71±0.50及纤维化9.11±2.03,组间比较差异有统计学意义(P<0.001,P<0.001,P<0.05)。正常组血清ALT为(40.82±8.74)U·L~(-1)、AST为(93.20±12.11)U·L~(-1)、TG为(0.60±0.17)mmol·L~(-1)、TC为(1.33±0.24)mmol·L~(-1),模型组分别为(112.12±23.40)U·L~(-1),(248.21±24.77)U·L~(-1),(0.78±0.21)mmol·L~(-1),(2.52±0.13)mmol·L~(-1),组间比较差异有统计学意义(P<0.001,P<0.001,P<0.05,P<0.001)。实验组的ALT(87.11±12.05)U·L~(-1)和AST(154.32±24.87)U·L~(-1),与模型组比较差异有统计学意义(P<0.01,P<0.001)。模型组血清IL-6为(151.38±30.67)pg·m L~(-1)、IL-8为(1.26±0.27)ng·m L~(-1)及TNF-α为(4.33±1.30)ng·mL~(-1),正常组分别为(66.83±15.08)pg·mL~(-1)、(0.71±0.27)ng·mL~(-1)、(1.46±0.45)ng·mL~(-1),组间比较差异有统计学意义(均P<0.001)。对照组IL-6为(110.17±23.60)pg·mL~(-1)、IL-8为(0.89±0.24)ng·mL~(-1)及TNF-α为(2.50±0.50)ng·mL~(-1),与模型组比较差异有统计学意义(均P<0.001);实验组IL-6为(100.42±15.10)pg·mL~(-1)、IL-8为(0.90±0.16)ng·mL~(-1)及TNF-α为(3.16±0.35)ng·mL~(-1),与模型组比较差异有统计学意义(P<0.001,P<0.001,P<0.01)。结论替米沙坦可降低非酒精性脂肪肝大鼠肝症反应及肝纤维化进程。
Objective To study the effects of telmisartan on inflammatory reaction and hepatic fibrosis in nonalcoholic fatty liver disease rats. Methods Male SD rats were fed with high-fat diet for 12 weeks and normal group (n = 10) fed with normal diet for 12 weeks. The model rats were randomly divided into 3 groups according to body weight: model group (n = 15, 1.0 mL · kg -1 · d -1 saline), control group (n = 10,8.4 mg · kg -1) (-1) · d -1 polyene phosphatidylcholine) and the experimental group (n = 10,8 mg · kg -1 -1 d -1 telmisartan), and continue Give high fat feed to feed. After 16 weeks, the rats were sacrificed and serum levels of ALT, AST, TC, IL-6 were measured by automatic biochemical analyzer. ), IL-8 and tumor necrosis factor-α (TNF-α) levels and liver histological changes in rats. Results The body weight of rats in the 16-week treatment group was (582.63 ± 33.59) g, and that in the normal group was (484.82 ± 33.69) g. There was significant difference between the two groups (P <0.001). The body weight of the control group and the experimental group were (538.54 ± 39.46) and (532.71 ± 40.85) g, respectively, with significant difference compared with the model group (P <0.01). The liver index of the model group was 4.01 ± 0.15, the normal group was 2.98 ± 0.29, the difference was statistically significant (P <0.00). The liver index of the control group was 3.54 ± 0.12, and the experimental group was 3.65 ± 0.13, which was significantly different from the model group (all P <0.001). Compared with the intra-articular lobular inflammation of 2.80 ± 0.42, the NSA score of 7.70 ± 0.48 and the fibrosis of 11.25 ± 2.12, the control group had intra-articular intra-articular inflammation of 1.42 ± 0.71, NSA score of 5.71 ± 0.50 and fibrosis of 9.11 ± 2.03, The difference was statistically significant (P <0.001, P <0.001, P <0.05). The serum ALT in the normal group was (40.82 ± 8.74) U · L -1, the AST was (93.20 ± 12.11) U · L -1 and the TG was (0.60 ± 0.17) mmol·L -1 , TC (1.33 ± 0.24) mmol·L -1 in the model group were (112.12 ± 23.40) U · L -1, (248.21 ± 24.77) U · L -1, 0.78 ± 0.21) mmol·L -1, (2.52 ± 0.13) mmol·L -1, there was significant difference between the two groups (P <0.001, P <0.001, P <0.05, P < 0.001). The levels of ALT (87.11 ± 12.05) U · L -1 and AST (154.32 ± 24.87) U · L -1 in the experimental group were significantly different from those in the model group (P <0.01, P <0.001) ). The level of IL-6 in the model group was (151.38 ± 30.67) pg · m L -1, the level of IL-8 was (1.26 ± 0.27) ng · m L -1 and the level of TNF-α was (4.33 ± 1.30) ng · mL -1 and (66.83 ± 15.08) pg · mL -1, (0.71 ± 0.27) ng · mL -1, (1.46 ± 0.45) ng · mL -1, (-1), the difference between the two groups was statistically significant (P <0.001). The levels of IL-6 in the control group were (110.17 ± 23.60) pg · mL -1, (0.89 ± 0.24) ng · mL -1 and (2.50 ± 0.50) ng · mL -1 (100.42 ± 15.10) pg · mL ~ (-1), IL-8 (0.90 ± 0.16) in the experimental group were significantly lower than those in the model group (all P <0.001) (ng · mL -1) and TNF-α (3.16 ± 0.35) ng · mL -1, respectively, with statistical significance (P <0.001, P <0.001, P <0.01) . Conclusion Telmisartan can reduce hepatic fibrosis and liver fibrosis in non-alcoholic fatty liver rats.