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研究比较了CTAB法和SDS法在枣和酸枣基因组DNA提取中的效果 ,并分析了取样时间、部位、样品状况、抗氧化剂和不同纯化处理等对所提DNA质和量及其RAPD扩增效果的影响。结果表明 :用改良后的CTAB法优于SDS法 ,可有效地去除多糖 ,所提DNA的质和量均能满足PCR扩增要求。取样时间与部位对所提基因组DNA的质量无影响 ,但与产量有关 ,以旺盛生长期的幼叶或嫩梢尖为佳。样品采后液氮处理 - 70℃低温保存 ,与新鲜材料所提DNA差异不大。抗氧化剂可有效地阻止多酚类物质氧化变褐 ,1%的 β 巯基乙醇即可满足要求。用于RAPD分析的模板DNA中含有RNA和少量蛋白质对扩增结果无影响
The effects of CTAB method and SDS method on the genomic DNA extraction of jujube and jujube were compared and the effects of sampling time, location, sample status, antioxidants and purification on DNA content and RAPD amplification were analyzed Impact. The results showed that the modified CTAB method was superior to SDS method, which could effectively remove polysaccharides. The quality and quantity of DNA could meet the requirements of PCR amplification. Sampling time and site have no effect on the quality of the genomic DNA mentioned, but it is related to the yield and it is better to use young leaves or shoot tips with strong growth period. After the sample liquid nitrogen treatment - 70 ℃ low temperature preservation, DNA and DNA extracted little difference. Antioxidants can effectively prevent oxidation and browning of polyphenols, 1% β-mercaptoethanol to meet the requirements. The template DNA used for RAPD analysis contained a small amount of RNA and protein had no effect on the amplification results