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目的研究强直性脊柱炎(AS)中HLA-B27抗原表达与基因亚型分布特点,探讨HLA-B27抗原和基因检测在AS诊断中的应用价值。方法收集103例AS患者及105例健康人群对照静脉全血,分别采用流式细胞术(FCM)及基于序列分析的PCR分型法(PCR-SBT)检测HLA-B27。结果 FCM检测HLA-B27诊断AS的灵敏度和特异度分别为97.09%和92.38%,PCR-SBT诊断AS的灵敏度和特异度为96.12%和97.14%。AS组HLA-B位点等位基因检出频率以B27最高。结论 HLA-B27抗原和基因检测对AS诊断均具有较高价值。FCM检出灵敏度略高于PCR-SBT,但特异度低于PCR-SBT。
Objective To investigate the distribution of HLA-B27 antigen and its distribution in ankylosing spondylitis (AS), and to explore the value of HLA-B27 antigen and gene detection in the diagnosis of AS. Methods A total of 103 AS patients and 105 healthy controls were enrolled in this study. HLA-B27 was detected by flow cytometry (FCM) and PCR-based sequencing (PCR-SBT). Results The sensitivity and specificity of FCM for the detection of AS in HLA-B27 were 97.09% and 92.38%, respectively. The sensitivity and specificity of PCR-SBT in diagnosing AS were 96.12% and 97.14%. The frequency of HLA-B locus allele in AS group was the highest in B27. Conclusion HLA-B27 antigen and gene test have high value in the diagnosis of AS. FCM detection sensitivity slightly higher than the PCR-SBT, but less than the specificity of PCR-SBT.