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目的 观察日本血吸虫病流行区人群的细胞免疫应答状态及吡喹酮治疗对其影响。方法 采集日本血吸虫病流行区12 9人 (粪检阳性者 6 4例 ,粪检阴性者 6 5例 ) ,吡喹酮治疗前及治疗后 45 d的静脉血 ,以血吸虫成虫和虫卵抗原分别刺激诱生细胞因子 ,检测培养上清中的细胞因子 IL- 5、IL- 10和 IFN- γ水平。结果 12 9例中 ,对血吸虫抗原刺激诱生的细胞因子水平粪检阴性组明显高于粪检阳性组 ;治疗后 ,细胞因子诱生水平较治疗前显著升高 ,特别是 IL- 5和 IFN- γ。结论 流行区人群的细胞免疫应答显示总体下调趋势 ;吡喹酮治疗后出现细胞因子水平的明显升高
Objective To observe the state of cellular immune response in schistosomiasis japonica population and the effect of praziquantel treatment on it. Methods A total of 129 schistosomiasis japonica endemic areas were enrolled in the study. Sixty-four were positive for feces and sixteen were for fecal negative. Pre-praziquantel and 45-day venous blood were collected. Cytokines were stimulated to induce cytokines IL-5, IL-10 and IFN-γ in culture supernatants. Results Among the 129 cases, the levels of cytokines induced by the antigen of schistosoma japonicum were significantly higher in the fecal negative group than those in the fecal positive group. After treatment, the levels of cytokines induced were significantly higher than those before treatment, especially IL-5 and IFN - γ. Conclusions The cellular immune responses in the endemic areas showed an overall downward trend; the levels of cytokines were significantly increased after praziquantel treatment