论文部分内容阅读
为了阐明人参在蒸制后不稳定皂甙是否发生变化,作者进行了红参皂甙的分离和鉴定。取南朝鲜专利局生产的红参甲醇提取之粗皂甙,经硅胶、硅烷化硅胶和反相高多孔聚合物联合柱层析后,得到如下已知皂甙(收率):R_0(0.04%)、R_(a1)(0.02%)、R_(a2)(0.03%)、R_(b1)(.38%)、R_(b2)(0.15%)、R_(b3)(0.01%)、Rc(0.14%)、Rd(0.04%)、Re(0.17%)、Rf(0.07%)、Rg_1(0.29%)和20-葡糖基人参甙Rf(0.008%)。这些皂甙的鉴定,是通过它们在不同TLC板上的Rf值、它们的乙酰化物或三甲硅烷(TMSi)醚的MS和~(13)C-NMR等数据与标准样品比较而被证实的。
In order to clarify whether ginseng changes after steaming unstable saponins, the authors carried out the separation and identification of ginsenosides. The crude saponin extracted from the red ginseng methanol extracted from the South Korean Patent Office was chromatographed on silica gel, silylated silica gel, and reversed-phase high-porous polymer to obtain the following known saponins (yield): R 0 (0.04%). R_(a1)(0.02%), R_(a2)(0.03%), R_(b1)(.38%), R_(b2)(0.15%), R_(b3)(0.01%), Rc(0.14% ), Rd (0.04%), Re (0.17%), Rf (0.07%), Rg_1 (0.29%) and 20-glucosyl ginseng Rf (0.008%). These saponins were identified by comparing their Rf values on different TLC plates, MS and 13C-NMR data of their acetylated or trimethylsilane (TMSi) ethers with standard samples.