目的：探讨逆转录病毒介导的反义ｂｃｌ２序列对细胞凋亡的诱导作用。方法：ＰＡ３１７细胞包装逆转录病毒，病毒转导Ｊｕｒｋａｔ细胞后用ＲＴＰＣＲ及Ｗｅｓｔｅｒｎ印迹法检测ｂｃｌ２ｍＲＮＡ及蛋白表达水平；用流式细胞仪计数及ＤＮＡ电泳检测细胞凋亡。结果：转染反义ｂｃｌ２序列可使内源性ＢＣＬ２蛋白表达下降，提高白血病细胞株对足叶乙甙（Ｖｐ１６）的敏感性，促进细胞凋亡。结论：反义ｂｃｌ２序列能促进Ｖｐ１６诱导的白血病细胞凋亡，为ｂｃｌ２反义技术治疗白血病的临床应用提供了实验依据。 Objective: To investigate the effect of retrovirus-mediated antisense bcl2 sequence on apoptosis. METHODS: PA317 cells were packaged with retroviruses, and Jurkat cells were transduced with the virus. The expression of bcl-2 mRNA and protein was detected by RT-PCR and Western blotting. Apoptosis was detected by flow cytometry and DNA electrophoresis. RESULTS: Transfection of antisense bcl2 sequence reduced the expression of endogenous BCL2 protein, increased the sensitivity of leukemic cell lines to Vp16, and promoted apoptosis. Conclusion: Antisense bcl2 sequence can promote the apoptosis of leukemia cells induced by Vp16, and provide experimental basis for the clinical application of bcl2 antisense technology in the treatment of leukemia.