论文部分内容阅读
目的 观察碱性成纤维细胞生长因子 (b FGF)能否通过胎盘屏障并干预宫内窘迫胎鼠脑损伤。 方法 15 d Wistar孕鼠 5只 ,经腹腔注射 1 2 5I标记的 b FGF(1 2 5I- b FGF) ,30 m in后取孕鼠血清、胎鼠脑、心、肝、脾、肺 ,检测各组织 1 2 5I- b FGF的放射活性 ;另取 32只怀孕 15 d Wistar孕鼠随机分为正常对照、窘迫对照、窘迫后干预及预防共四组 ,b FGF干预 ,免疫荧光双标记法标记增殖的神经元细胞。 结果 孕鼠注射 1 2 5I- b FGF 15 0 ng/ g后 ,胎脑、胎肝及胎心之 1 2 5I- b FGF分别为 (3.5± 2 .1)、(7.3± 4 .9)、(12 .1± 4 .6 ) ng/ g,差异有显著性 (t>2 .5 8,P<0 .0 1) ;各组增殖神经元计数分别为 (4 .5± 2 .4 )、(5 .8± 3.1)、(17.2± 5 .4 )、(18.1± 5 .8)个 / HP,差异有非常显著性 (F =12 8,P<0 .0 0 1) ;各组存活神经元总数分别为 (6 5± 9)、(30± 8)、(5 6± 8)、(5 9± 7)个 / HP,差异有非常显著性 (F=12 8,P<0 .0 0 1)。 结论 b FGF能够通过胎盘屏障并促进宫内窘迫胎鼠神经元增殖并增加神经元存活数。
Objective To observe whether bFGF can pass through the placental barrier and interfere with intrauterine fetal distress fetal brain injury. Methods Fifteen pregnant Wistar pregnant rats were injected intraperitoneally with 125 F-labeled bFGF (125I-b FGF). After 30 m injections, serum, fetal rat brain, heart, liver, spleen and lung In addition, 32 Wistar pregnant rats were randomly divided into normal control, distress control, post-distress intervention and prevention of four groups, b FGF intervention, immunofluorescence double labeling method Proliferating neuronal cells. RESULTS: After the pregnant rats were injected with 150 ng / g 125 ng / g FGF, their fetal brain, fetal liver and fetal heart rate were (3.5 ± 2 .1), (7.3 ± 4. 9), (12.1 ± 4.6) ng / g, the difference was significant (t> 2.58, P <0.01). The proliferation neuron counts in each group were (4.5 ± 2. 4) , (5 .8 ± 3.1), (17.2 ± 5 .4) and (18.1 ± 5. 8) / HP respectively. The differences were significant (F = 12.8, P <0.01) The total number of surviving neurons were (65 ± 9), (30 ± 8), (56 ± 8) and (59 ± 7) / HP, respectively, with significant difference .0 0 1). Conclusion b FGF can cross the placental barrier and promote intrauterine fetal distress fetal neuronal proliferation and increase neuronal survival.