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目的研究蛇足石杉Huperzia serrata的化学成分。方法利用硅胶柱色谱法、Sephadex LH-20凝胶柱色谱法、中压柱色谱及半制备高效液相色谱等方法分离纯化;通过核磁共振谱、质谱等光谱数据鉴定化合物结构。结果分离得到10个化合物,分别鉴定为β-谷甾醇(1)、5,7,4′-三羟基-3′-甲氧基黄酮(2)、5,7,4′-三羟基-3′,5′-二甲氧基黄酮(3)、芹菜素(4)、正三十烷醇(5)、21β-hydroxy-serrat-14-en-3β-ol(6)、21α-hydroxy-serrat-14-en-3β-ol(7)、16-oxo-21β-hydroxy-serrat-14-en-3α-yl-acetate(8)、3β,21α-dihydroxy-serrat-14-en-24-ol(9)、3α,21β-dihydroxy-serrat-14-en-24-ol(10)。结论化合物2～5为首次从本植物中分离得到。
Objective To study the chemical constituents of Huperzia serrata. Methods The compounds were isolated and purified by silica gel column chromatography, Sephadex LH-20 column chromatography, medium pressure column chromatography and semi-preparative high performance liquid chromatography. The structures of the compounds were identified by NMR, MS and other spectral data. Results Ten compounds were isolated and identified as β-sitosterol (1), 5,7,4’-trihydroxy-3’-methoxyflavone (2), 5,7,4’-trihydroxy-3 (3), apigenin (4), n-triacontanol (5), 21β-hydroxy-serrat-14-en- ser-14-en-3β-ol (7), 16-oxo-21β-hydroxy-serrat- ol (9), 3α, 21β-dihydroxy-serrat-14-en-24-ol (10). Conclusion Compounds 2 ~ 5 were isolated from this plant for the first time.