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建立了高效液相色谱法监测人口服辛伐他汀药物后的血药浓度。血样用环己烷 -二氯甲烷 (体积比为 3.5∶ 1)提取 ,以洛伐他汀为内标 ,在 2 37nm波长下检测 ;色谱柱 :Lichrospher C1 8( 2 0 0 mm× 4 .6mm i.d.,5μm ) ,流动相 :乙腈 -水 (体积比为 70∶ 30 ) ;流速 :1.2 m L/ m in。血药浓度在 0 .2 5～ 50 .0μg/ L范围内与峰面积和内标峰面积的比值之间线性关系良好 ,日内及日间相对标准偏差 ( n =5)分别低于 7.94 %和 8.58% ,回收率高于93.3%。方法灵敏、准确、快速 ,适用于药物动力学和药效学研究
A high performance liquid chromatography (HPLC) was developed to monitor plasma concentration of human simvastatin. Blood samples were extracted with cyclohexane-methylene chloride (3.5: 1 by volume), lovastatin was used as an internal standard and detected at 2 37nm. Column: Lichrospher C1 8 (200 mm × 4.6mm id , 5μm), mobile phase: acetonitrile-water (volume ratio 70:30); flow rate: 1.2 m L / min. The linearity of plasma concentration ranged from 0.52 to 50.0 μg / L to peak area and internal standard peak area, and the relative standard deviations (n = 5) were lower than 7.94% and 8.58%, the recovery rate is higher than 93.3%. The method is sensitive, accurate and rapid and suitable for pharmacokinetic and pharmacodynamic studies