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目的研究维甲类化合物Ro40 875 7对人胰腺癌JF 30 5细胞株的抗增殖作用及其作用机制。方法采用四甲基氮唑蓝实验、流式细胞技术、Westernblot蛋白检测技术分别检测Ro40 875 7对人胰腺癌JF 30 5细胞株的增殖、细胞周期和p2 7蛋白表达的影响。结果Ro40 875 7可明显抑制JF 30 5细胞株的生长 ,并呈剂量、时间依赖性作用。用 10 - 5mol LRo40 875 7处理细胞 72h ,G1 期细胞由 5 6 %增加至 76 % ,S期细胞由 2 5 %减少至 7% ,细胞周期阻滞于G1 期 ;用 10 - 5mol LRo40 875 7处理12h ,JF 30 5细胞p2 7蛋白表达增加至对照组的 2 2倍、2 4h为 1 9倍 ,48h为 1 2倍、72h为 0 7倍。结论Ro40 875 7对人胰腺癌JF 30 5细胞株具有明显的生长抑制作用 ,并可以导致其G1 期细胞周期阻滞和上调p2 7蛋白表达。上调p2 7蛋白引起细胞周期阻滞可能是其抗肿瘤的机制之一
Objective To study the antiproliferative effect of retinoid Ro40 875 7 on human pancreatic cancer JF 30 5 cell line and its mechanism. Methods The effects of Ro40 875 7 on the proliferation, cell cycle and p27 protein expression of human pancreatic cancer JF-30 cell line were detected by MTT assay, flow cytometry and Western blotting. Results Ro40 875 7 could significantly inhibit the growth of JF 30 5 cell line in a dose-and time-dependent manner. Cells were treated with 10 - 5mol LRo40 875 7 for 72h. The percentage of cells in G1 phase increased from 56% to 76% in S phase and from 25% to 7% in S phase. The cell cycle was arrested in G1 phase. Cells were treated with 10 - 5mol LRo40 875 7 At 12 h, the expression of p27 protein in JF305 cells increased to 2 2 times of that of the control group, 1 9 times at 24 hours, 12 times at 48 hours and 0 7 times at 72 hours. Conclusion Ro40 875 7 has obvious growth inhibitory effect on human pancreatic cancer JF 30 5 cell line and can lead to cell cycle arrest in G1 phase and up-regulation of p27 protein expression. Upregulation of p27 protein causes cell cycle arrest may be one of its anti-tumor mechanisms