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本研究中我们分离骨髓和脂肪的间充质干细胞,分别检测它们对T细胞周期、活化、抑制和增殖的作用情况,研究脂肪来源(adipose-derived mesenchemal stem cell,AMSC)和骨髓来源(bone marrow derived mesenchemal stem cell,BMSC)间充质干细胞的免疫学特征,比较它们是否存在免疫功能的差异,结果显示AMSC和BMSC均具有抑制T细胞增殖的能力,在有丝分裂原刺激和MLR的T细胞增殖中均具有剂量依赖性的,在1∶2时有极强的抑制作用,但是在1∶100时这种作用基本消失,在共培养时AMSC和BMSC都可使更多的T细胞被抑制在G0/G1期,同时也可以抑制T细胞的早期活化,但是上述作用AMSC均较BMSC弱,但是AMSC并不具有抑制T细胞凋亡的作用,而两者在Th0向Th1或Th2分化中所起的作用是基本相似的,主要抑制Th0向Th1细胞(IL-2和IFN-γ生成细胞)的分化,而对向Th2细胞(IL-4和IL-10生成细胞)分化没有明显的影响。所以,AMSC和BMSC具有类似的免疫调节作用,因AMSC取材方便而成为更佳的材料来源。
In this study, we isolated bone marrow and adipose-derived mesenchymal stem cells and examined their effects on the cycle, activation, inhibition and proliferation of T cells, respectively. We investigated the effects of adipose-derived mesenchemal stem cells (AMSCs) and bone marrow derived mesenchymal stem cells (BMSC)). The immunological characteristics of mesenchymal stem cells (BMSC) were compared. The differences of immune function between them were observed. Both AMSC and BMSC had the ability to inhibit the proliferation of T cells. In both mitogen-stimulated and MLR T cell proliferation All in a dose-dependent manner with strong inhibition at 1: 2, but disappeared almost at 1: 100. Both AMSC and BMSC inhibited more T cells in G0 during co-culture / G1 phase, but also can inhibit the early activation of T cells, but the role of AMSC than BMSC are weaker, but AMSC does not have the effect of inhibiting T cell apoptosis, and both Th0 Th1 or Th2 differentiation The effect is essentially similar and mainly inhibits the differentiation of Th0 into Th1 cells (IL-2 and IFN-γ producing cells) without significant effect on the differentiation of Th2 cells (IL-4 and IL-10 producing cells). Therefore, AMSC and BMSC have a similar immunomodulatory effect, which makes AMSC a better source of material because of the convenience of AMSC.