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为了优化将抗体偶联在二氧化硅试管表面上以便进行丙型肝炎抗原检测的分析系统,本研究通过氨基硅烷的活化作用,在玻璃表面形成活化的氨基,以戊二醛作为化学交联试剂,在已硅化的玻璃表面固定丙肝单克隆抗体,并进行丙肝抗原(HCAg)的测定。结果显示,通过条件优化实验,发现以10%(V/V)的氨基硅烷水溶液处理玻璃试管3h后,再用3%(V/V)的戊二醛水溶液交联丙肝单克隆抗体2h,可以得到固定效果较好,非特异性较低的玻璃载体,对HCAg可测至1μg/L。可见,用该方法制备的玻璃载体可为进一步建立新的HCAg磁性免疫检测系统提供理论和实验依据。
In order to optimize the analytical system for coupling antibodies on the surface of silica tubes for the detection of hepatitis C antigens, activated amino groups were formed on the surface of the glass by the activation of aminosilanes, and glutaraldehyde was used as a chemical crosslinking reagent , Immobilized on the siliconized glass surface of monoclonal antibodies to hepatitis C, and hepatitis C antigen (HCAg) determination. The results showed that after the glass tube was treated with 10% (V / V) aminosilane aqueous solution for 3 h, the monoclonal antibody of hepatitis C was cross-linked with 3% (V / V) glutaraldehyde for 2 h by conditional optimization experiments. Get a fixed effect is better, non-specific lower glass carrier, HCAg measurable to 1μg / L. It can be seen that the glass carrier prepared by the method can provide theoretical and experimental basis for further establishing a new magnetic immunity detection system of HCAg.