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目的研究白细胞介素１β（ＩＬ－１β）和白细胞介素８（ＩＬ－８）在结肠炎发病中的作用，观察白细胞介素－１受体拮抗剂（ＩＬ－１ｒａ）对结肠炎的疗效。方法用３０ｍｇ三硝基苯磺酸（ＴＮＢＳ）＋０．２５ｍｌ体积分数为５０％乙醇制成大鼠慢性实验性结肠炎模型，然后于不同时间点尾静脉注入７ｍｇ／ｋｇＩＬ－１ｒａ，进行实验性治疗，以静脉注入３ｍｇ氢化可的松琥珀酸钠作为治疗对照，同时观察治疗前后病变结肠组织学变化，测定结肠髓过氧化物酶（ＭＰＯ）和超氧化物歧化酶（ＳＯＤ）活性改变，以原位杂交法观察结肠道组织ＩＬ－１β和ＩＬ－８ｍＲＮＡ表达水平。结果模型组大鼠结肠组织均出现粘膜下充血、水肿、出血及炎症细胞浸润、粘膜溃疡和隐窝脓肿，以致炎后３ｄ为著，７ｄ后减轻，２１ｄ基本恢复正常。模型组二次致炎后上述表现再次出现。整个炎症期均有ＩＬ－１βｍＲＮＡ表达，而ＩＬ－８ｍＲＮＡ表达出现于致炎后３ｄ。两种白细胞介素ｍＲＮＡ的表达部位均主要在粘膜固有层和粘膜下层的单核巨噬细胞，但在致炎后３ｄ内，ＩＬ－１βｍＲＮＡ在肠上皮细胞也有表达。ＩＬ－１ｒａ治疗后３组大鼠结肠炎症均明显减轻，伴随相应的ＭＰＯ下降和ＳＯＤ上升，两种白细胞介素ｍＲＮＡ的表达均未?
Objective To investigate the role of interleukin-1β (IL-1β) and interleukin-8 (IL-8) in the pathogenesis of colitis and to investigate the effect of interleukin-1 receptor antagonist (IL-1ra) on colitis. Methods Chronic experimental colitis model was induced in rats by adding 30mg trinitrobenzene sulfonic acid (TNBS) + 0.25ml volume fraction of 50% ethanol, then injecting 7mg / kg IL-1ra intravenously at different time points for experimental treatment , Intravenous infusion of 3mg hydrocortisone sodium succinate as a treatment control, and observed before and after treatment of lesions in the histological changes measured colonic myeloperoxidase (MPO) and superoxide dismutase (SOD) activity changes to the original The hybridization method was used to observe the expression of IL-1β and IL-8 mRNA in colon tissue. Results In the model group, submucosal congestion, edema, hemorrhage, infiltration of inflammatory cells, mucosal ulceration and crypt abscess occurred in the colon tissues of rats in the model group. The rats in the model group were on the 3d after the inflammation, relieved on the 7th day, and returned to normal on the 21st day. The model group after secondary inflammation of the above performance again. IL-1βmRNA expression was observed in the entire inflammatory phase, while IL-8mRNA expression appeared 3d after pro-inflammatory. Both IL-2 mRNA expression sites were mainly in monolayer and submucosal mononuclear macrophages, but IL-1β mRNA was also expressed in intestinal epithelial cells within 3d after pro-inflammatory. IL-1ra treatment of colitis in rats after 3 groups were significantly reduced, accompanied by a corresponding decline in MPO and SOD increased, the two interleukin mRNA expression were not?