论文部分内容阅读
以自贡冬豆为试材,采用自来水和固体培养基对大豆组培再生枝进行生根诱导培养,测定了两种培养条件下的生根速率、根数量、根长度及移栽到土壤后植株的成活率,以期为组培苗生根方法的改进提供参考。结果表明:大豆再生枝在自来水培养条件下仅4 d就开始生根,而在固体培养基中则大约需要7 d才开始生根。培养28 d后,自来水中再生枝的生根数为5.26±3.40条.株-1,平均根长为6.29±4.82 cm(n=60);而固体培养基中再生枝的生根数达19.54±11.50条.株-1(n=60),但平均根长仅为1.97±0.82 cm(n=60)。再生植株移栽后,自来水培养再生苗的成活率明显高于固体培养基,两种植株成熟后的结荚数及种子质量没有明显差异。由于再生枝在自来水中培养有助于缩短培养周期,减少污染,提高生根效率,降低培养成本,因此自来水在某些大豆品种的组织培养中可以代替固定的生根培养基。
Taking Zigong winter bean as test material, the regenerated shoots of soybean tissue culture were induced by tap water and solid medium. The rooting rate, root number, root length and the survival of the plants after transplanting to soil were measured Rate, in order to provide a reference for the improvement of rooting method of tissue culture seedlings. The results showed that the regenerated shoots of soybean began to root in only 4 days in tap water culture, while in solid medium it took about 7 days to start rooting. After 28 days of culture, the rooting number of regenerated branches in tap water was 5.26 ± 3.40, the average root length was 6.29 ± 4.82 cm (n = 60), while the number of regenerated branches in solid medium was 19.54 ± 11.50 Strain -1 (n = 60), but the average root length was only 1.97 ± 0.82 cm (n = 60). After transplanting the regenerated plants, the survival rate of regenerated seedlings in tap water was significantly higher than that in solid medium. There was no significant difference in the pod numbers and seed quality between the two plants after maturing. Because regenerated shoots are cultured in tap water to help shorten the incubation period, reduce pollution, improve rooting efficiency and reduce culture costs, tap water can replace fixed rooting medium in the tissue culture of some soybean varieties.