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目的探讨有限稀释定量ＰＣＲ检测血清ＨＢＶ－ＤＮＡ对干扰素抗病毒治疗的指导意义。方法利用有限稀释定量ＰＣＲ技术分别检测 ３８例 ＨＢｓＡｇ、ＨＢｅＡｇ双阳性患者 α－２ｂ干扰素治疗前后血清 ＨＢＶ－ＤＮＡ含量。结果 ３８例患者干扰素治疗 １２周 ＨＢＶ－ＤＮＡ阴转７例（１８．４％）；治疗２４周阴转１１例（２８．９％）。其中病毒滴度＞２５０ｐｇ／ｍＬ者阴转１１．１％（１／９）；病毒滴度２５ｆｇ～２５０ｐｇ／ｍＬ者阴转２５．０％（４／１６）；病毒滴度＜２５ｆｇ／ｍＬ者阴转４６．２％（６／１３），三组比较Ｐ＜０．０１。结论有限稀释定量ＰＣＲ检测ＨＢＶ－ＤＮＡ对慢性乙型肝炎患者干扰素治疗具有一定的指导意义，以病毒滴度≤２５０ｐｇ／ｍＬ、干扰素治疗２４周疗效较佳。
Objective To investigate the significance of limiting dilution quantitative PCR in detecting serum HBV-DNA for interferon antiviral therapy. Methods The serum HBV-DNA levels of 38 patients with HBsAg and HBeAg double positive before and after interferon α-2b treatment were detected by limited dilution quantitative PCR. Results 38 patients were treated with interferon for 12 weeks and 7 cases (18.4%) were negative for HBV-DNA, while 11 cases (28.9%) were negative for 24 weeks. The virus titers> 250pg / mL were negative 11.1% (1/9); virus titers 25fg ~ 250pg / mL were negative 25.0% (4/16); virus titers <25fg / mL Negative 46.2% (6/13), three groups P <0.01. Conclusion Finite-dilution quantitative polymerase chain reaction (PCR) detection of HBV-DNA is of guiding significance for the treatment of interferon in patients with chronic hepatitis B. The titer of virus is less than 250 pg / mL, and the effect of interferon for 24 weeks is better.