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【目的】鉴定稻纵卷叶螟Cnaphalocrocis medinalis的羧酸酯酶基因,并检测这些基因在成虫不同组织中的表达模式。【方法】从稻纵卷叶螟转录组中搜索羧酸酯酶基因,使用生物信息学软件对所获得的基因序列进行分析,使用荧光定量PCR检测这些基因在各组织中的相对表达水平。【结果】获得了15个羧酸酯酶基因,分别命名为Cm Car E1~Cm Car E15。其中Cm Car E12缺少3′区域,其余14个序列均含有完整的开放阅读框。除Cm Car E11外,其余14个基因编码的蛋白均具有羧酸酯酶的典型特征,如保守的五肽结构域,催化三联体和氧阴离子穴等。系统进化分析显示15个Cm Car Es被聚在不同的进化支内,Cm Car E13、Cm Car E14和Cm Car E15聚在“胞内催化类”进化支,其余12个Cm Car Es聚在“分泌催化类”进化支。Cm Car E1、Cm Car E2、Cm Car E3、Cm Car E7、Cm Car E8、Cm Car E10和Cm Car E13特异表达于成虫腹部,而Cm Car E9特异表达于雌雄成虫触角。其他基因的表达没有组织特异性。【结论】Cm Car E1、Cm Car E2、Cm Car E3、Cm Car E7、Cm Car E8、Cm Car E10和Cm Car E13编码的酯酶可能参与了内外源化合物的代谢,而Cm Car E9编码的酯酶可能参与了气味分子的降解。
【Objective】 The objective of this study was to identify the carboxylesterase genes of Cnaphalocrocis medinalis and to examine the expression pattern of these genes in different tissues of adult adults. 【Method】 Carboxylesterase genes were searched from the rice leaf roller transcriptome. Bioinformatics software was used to analyze the gene sequences. The relative expression levels of these genes were detected by fluorescence quantitative PCR. 【Result】 Fifteen carboxylesterase genes were obtained and named Cm Car E1 ~ Cm Car E15 respectively. Among them, Cm Car E12 lacks the 3 ’region and the remaining 14 sequences contain a complete open reading frame. In addition to Cm Car E11, the other 14 genes encoded by the protein carboxylesterase have typical characteristics, such as the conserved pentapeptide domain, catalytic triad and oxygen anion hole and so on. Phylogenetic analysis showed that 15 Cm Car Es clustered in different clades, Cm Car E13, Cm Car E14 and Cm Car E15 clustered in the “intracellular catalysis” clade and the remaining 12 Cm Car Es clustered in “Secretion catalysis ” clade. Cm Car E1, Cm Car E2, Cm Car E3, Cm Car E7, Cm Car E8, Cm Car E10 and Cm Car E13 were specifically expressed in the abdomen of adult adults, while Cm Car E9 was specifically expressed in the antennae of both male and female adults. The expression of other genes is not tissue-specific. 【Conclusion】 The esterase encoded by Cm Car E1, Cm Car E2, Cm Car E3, Cm Car E7, Cm Car E8, Cm Car E10 and Cm Car E13 may be involved in the metabolism of endogenous and exogenous compounds, whereas Cm Car E9 encoded ester Enzymes may be involved in the degradation of odor molecules.