【目的】了解新疆小麦材料多酚氧化酶(PPO)活性基因的等位变异组成和分布以及与PPO活性的关系,为小麦PPO活性的分子标记辅助选择奠定基础。【方法】利用PPO基因的功能标记PPO18、PPO29和PPO16对445份新疆冬春小麦材料进行2A和2D染色体上PPO等位基因Ppo-A1a、Ppo-A1b、Ppo-D1a和Ppo-D1b的检测,分析不同等位变异与PPO活性的相关性及变化趋势。【结果】PPO18在等位基因Ppo-A1a(高PPO)和Ppo-A1b(低PPO)中的分布频率为73.03%和26.97%,PPO16和PPO29在等位基因Ppo-D1a(低PPO)和Ppo-D1b(高PPO)中的分布频率76.63%和23.37%。Ppo-A1a与Ppo-A1b基因型的PPO活性平均值差异达极显著水平(P<0.01)。而Ppo-D1a与Ppo-D1b两者基因型的平均PPO活性差异不显著(P0.05);两个PPO基因的等位基因组合类型分布频率为:Ppo-A1a/Ppo-D1a(54.38%)、Ppo-A1a/Ppo-D1b(18.65%)、Ppo-A1b/Ppo-D1a(22.25%)和Ppo-A1b/Ppo-D1b(4.72%);同时冬春小麦材料间PPO活性基因分布存在明显差异,总体来看,新疆小麦材料中高PPO活性的等位变异类型所占比例较高。【结论】PPO18、PPO16和PPO29标记可以作为小麦材料PPO活性鉴定有效工具。可直接利用此标记进行标记辅助选择。 【Objective】 The objective of this study is to understand the allelic variation of PPO activity and its relationship with PPO activity in Xinjiang and lay a foundation for molecular marker-assisted selection of wheat PPO activity. 【Method】 The PPO alleles Ppo-A1a, Ppo-A1b, Ppo-D1a and Ppo-D1b on chromosomes 2A and 2D were detected using PPO18, PPO29 and PPO16 markers in 445 Xinjiang winter and spring wheat cultivars. The correlation and trend of different alleles with PPO activity were analyzed. 【Results】 The distribution frequencies of PPO18 in Ppo-A1a (high PPO) and Ppo-A1b (low PPO) were 73.03% and 26.97%, respectively. The frequencies of PPO16 and PPO29 in alleles Ppo-D1a -D1b (high PPO) distribution frequency of 76.63% and 23.37%. The average difference of PPO activity between Ppo-A1a and Ppo-A1b genotypes reached significant level (P <0.01). The average PPO activity of both Ppo-D1a and Ppo-D1b genotypes was not significantly different (P> 0.05). The frequency of allele combinations of the two PPO genes was Ppo-A1a / Ppo-D1a (54.38% , Ppo-A1a / Ppo-D1b (18.65%), Ppo-A1b / Ppo-D1a (22.25%) and Ppo-A1b / Ppo-D1b In general, the proportion of allelic variants with high PPO activity in Xinjiang wheat was higher. 【Conclusion】 The markers PPO18, PPO16 and PPO29 can be used as effective tools for the identification of PPO activity in wheat. This marker can be used directly for marker-assisted selection.