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研究了辣椒疫霉对双炔酰菌胺的敏感性及其抗药性风险。采用菌丝生长速率法测定了分别采自河北定兴、藁城和山东寿光、青州等地的173株辣椒疫霉菌株对双炔酰菌胺的敏感性,结果表明,其EC50值介于0.002 7～0.049 9μg/mL之间,平均EC50值为0.021 9±0.010 2μg/mL,敏感性呈单峰曲线分布;采用紫外诱导+菌落角变和药剂驯化的方法分别处理10个供试菌株,各获得7株和1株突变体,其抗性倍数在41.50～394 437.77之间,突变频率分别为1.46%和1.43%。对突变体部分生物学性状的研究表明,抗性突变体与其亲本菌株在菌丝生长速率、致病性、产孢能力、适合度指数和竞争力方面均具有不同程度的差异;抗性突变体DX 2-3 20m和DX 2-3 F7的产孢量、适合度和竞争力均高于亲本菌株,有利于抗性群体的形成。研究表明,辣椒疫霉对双炔酰菌胺存在中等抗性风险。
The susceptibility and resistance of Phytophthora capsici to mandipropamid were studied. The mycelial growth rate method was used to determine the susceptibility of 173 capsici strains to mandipropamid in Dingxing, Yuncheng, Shouguang, Qingzhou, Hebei and other places. The results showed that the EC50 values ranged from 0.002 The average EC50 value was 0.021 9 ± 0.010 2 μg / mL, and the sensitivity was single peak curve distribution. Ten tested strains were treated with UV-induced + colonization and drug domestication respectively Seven strains and one mutant were obtained, with the resistance multiples ranging from 41.50 to 394 437.77. The mutation frequencies were 1.46% and 1.43%, respectively. The research on some biological characters of mutants showed that the resistant mutants and their parental strains had different degrees of mycelial growth rate, pathogenicity, sporulation ability, fitness index and competitiveness. The resistant mutants The sporulation, fitness and competitiveness of DX 2-3 20m and DX 2-3 F7 were higher than that of the parental strain, which was in favor of the formation of resistant population. Studies have shown that Phytophthora capsici has a moderate resistance to mandipropamid.