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Soybean frog-eye leaf spot(FLS) has been a major disease andproduction constralnt in the Northeast of China.The paper reported methods for identifying and evaluating resistant germplasm to the disease ,at sometime listed several resistant cultivars or lines which were resistant to both dominant races (1,7 race)and the mixture of over 8 races.It indicated that the resistance to race 1 or 7 were due to two different single dominant genes ,respectively.And in the fled,there were more races,the resistance showed quantitative character.It was proposed the LTP,which was made according to the sizeand number of lesions could be used as an important index in selecting.Using bulked segregants analysis (BSA),the genetic distance between OPSO3620 and resistant gene to race 7 were identified 8.7cm.The results showed that the resistant cultivars had less number of stoma ,closer arrangement of polisade tissue and high cotent of wax.